Evidence map›Paper›PMID 24879917›Full record

Trial reportGene2014

The effect of Helicobacter pylori CagA on the HER-2 copy number and expression in gastric cancer.

Jung Ho Shim, Jung Hwan Yoon, Sung Sook Choi, Hassan Ashktorab, Duane T Smoot, Kyo Young Song, Suk Woo Nam, Jung Young Lee, Cho Hyun Park, Won Sang Park

Abstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Gene, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Eradication ofFrontiers in microbiology · 2021
    Review
  7. Article
  8. Review
  9. Host pathogen interactions inWorld journal of gastroenterology · 2017
    Review
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Jung Ho ShimDivision of Gastrointestinal Surgery, Department of Surgery, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
Jung Hwan YoonDepartment of Pathology, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
Sung Sook ChoiCollege of Pharmacy, Sahmyook University, Hwarangro 815, Nowon-gu, Seoul 139-742, South Korea.
Hassan AshktorabDepartment of Medicine, Howard University, Washington, DC 20060, USA.
Duane T SmootDepartment of Medicine, Howard University, Washington, DC 20060, USA.
Kyo Young SongDivision of Gastrointestinal Surgery, Department of Surgery, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
Suk Woo NamDepartment of Pathology, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
Jung Young LeeDepartment of Pathology, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
Cho Hyun ParkDivision of Gastrointestinal Surgery, Department of Surgery, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
Won Sang ParkDepartment of Pathology, College of Medicine, The Catholic University of Korea, Seoul, South Korea. Electronic address: wonsang@catholic.ac.kr.
Catholic University of Korea · KRHoward University · USSahmyook University · KR

Funding

PROTEOMICS CORE FACILITYG12MD007597 · NIMHD · HOWARD UNIVERSITY · PI SOUTHERLAND, WILLIAM M. · 2012 to 2018
$14.8M
NIMHD NIH HHS G12 MD007597
6 · The paper itself

Abstract

We investigated whether Helicobacter pylori (H. pylori) CagA contributes to the DNA copy change and mRNA transcript expression of the HER-2 gene and, consequently, affects HER-2 protein expression to evaluate the significance of CagA and HER-2 amplification in gastric cancer. We used the AGS and MKN1 gastric cancer and HFE-145 immortalized non-neoplastic gastric mucosa cell lines. We also confirmed the effects of CagA on HER-2 expression in human gastric cancer tissues and gastric mucosal tissues of H. pylori infected C57BL/6 mice. Ectopic CagA expression in AGS, MKN1 and HFE-145 cells showed a significant increase in HER-2 gene copy number and expression. The gastric mucosae of H. pylori infected C57BL/6 mice also showed increased HER-2 DNA copy number and protein expression. In addition, CagA expression was detected in 17 (56.7%) of 30 gastric cancer tissues, and eight (47%) of them showed HER-2 DNA amplification of more than two-fold. In immunohistochemistry, HER-2 overexpression was detected in 12 (40%) of 30 gastric cancers and a positive correlation was observed among DNA copy number, the mRNA transcript, and protein expression of the HER-2 gene in gastric cancer (P<0.05). These results suggest that H. pylori CagA may induce overexpression of the HER-2 protein by increasing HER-2 DNA and mRNA copy number.

Indexed as

Gene DosageGene Expression Regulation, NeoplasticAnimalsAntigens, BacterialBacterial ProteinsCell Line, TumorErb-b2 Receptor Tyrosine KinasesFemaleGastric MucosaHelicobacter pyloriHumansMaleMiceRNA, MessengerRNA, NeoplasmStomach NeoplasmsAntigens, BacterialBacterial ProteinscagA protein, Helicobacter pyloriERBB2 protein, humanErbb2 protein, mouseErb-b2 Receptor Tyrosine KinasesRNA, MessengerRNA, NeoplasmCagADNA amplificationGastric cancerHER-2/neuH. pylori

Identifiers

PMID24879917
PMCPMC4286173
OpenAlexW2070978992

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.