ArticleMammalian genome : official journal of the International Mammalian Genome Society2014
Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system.
Article in Mammalian genome : official journal of the International Mammalian Genome Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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60 citing papers in PubMed, 115 citations in OpenAlex.
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- VDGE: a data repository of variation database for gene-edited animals across multiple species.Nucleic acids research · 2025Article
- Generation of albino C57BL/6J mice by CRISPR embryo editing of the mouse tyrosinase locus.Journal of genetics · 2025Article
- Mitochondrial biogenesis in white adipose tissue mediated by JMJD1A-PGC-1 axis limits age-related metabolic disease.iScience · 2024Article
- A universal method for generating knockout mice in multiple genetic backgrounds using zygote electroporation.Biology open · 2023Article
- CRISPR-Cas Technology as a Revolutionary Genome Editing tool: Mechanisms and Biomedical Applications.Iranian biomedical journal · 2023Review
- Successful induction of pseudopregnancy using sonic vibration in mice.Scientific reports · 2023Article
- Expanding the genome editing toolbox of Saccharomyces cerevisiae with the endonuclease ErCas12a.FEMS yeast research · 2023Article
- Genotyping Genome-Edited Founders and Subsequent Generation.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Amount of Cas9 protein introduced into mouse embryos via electroporation affects the genome-editing rate.The Journal of reproduction and development · 2022Article
- Genetic quality: a complex issue for experimental study reproducibility.Transgenic research · 2022Review
- MYPT1-PP1β phosphatase negatively regulates both chromatin landscape and co-activator recruitment for beige adipogenesis.Nature communications · 2022Article
- Coordination chemogenetics for activation of GPCR-type glutamate receptors in brain tissue.Nature communications · 2022Article
- CRISPR/Cas9-Mediated Transgenesis of the Masu Salmon (Oncorhynchus masou) elovl2 Gene Improves n-3 Fatty Acid Content in Channel Catfish (Ictalurus punctatus).Marine biotechnology (New York, N.Y.) · 2022Article
- Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci.BMC biotechnology · 2022Article
- Distinctive High Expression of Antiretroviral APOBEC3 Protein in Mouse Germinal Center B Cells.Viruses · 2022Article
- DAJIN enables multiplex genotyping to simultaneously validate intended and unintended target genome editing outcomes.PLoS biology · 2022Article
- Efficient CRISPR Mutagenesis in Sturgeon Demonstrates Its Utility in Large, Slow-Maturing Vertebrates.Frontiers in cell and developmental biology · 2022Review
- Albino mice with the point mutation at the tyrosinase locus show high cholesterol diet-induced NASH susceptibility.Scientific reports · 2021Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single nucleotide mutations (SNMs) are associated with a variety of human diseases. The CRISPR/Cas9 genome-editing system is expected to be useful as a genetic modification method for production of SNM-induced mice. To investigate whether SNM-induced mice can be generated by zygote microinjection of CRISPR/Cas9 vector and single-stranded DNA (ssDNA) donor, we attempted to produce albino C57BL/6J mice carrying the Tyr gene SNM (G291T) from pigmented C57BL/6J zygotes. We first designed and constructed a CRISPR/Cas9 expression vector for the Tyr gene (px330-Tyr-M). DNA cleavage activity of px330-Tyr-M at the target site of the Tyr gene was confirmed by the EGxxFP system. We also designed an ssDNA donor for homology-directed repair (HDR)-mediated gene modification. The px330-Tyr-M vector and ssDNA donor were co-microinjected into the pronuclei of 224 one-cell-stage embryos derived from C57BL/6J mice. We obtained 60 neonates, 28 of which showed the ocular albinism and absence of coat pigmentation. Genomic sequencing analysis of the albino mice revealed that the target of SNM, G291T in the Tyr gene, occurred in 11 mice and one founder was homozygously mutated. The remaining albino founders without Tyr G291T mutation also possessed biallelic deletion and insertion mutants adjacent to the target site in the Tyr locus. Simple production of albino C57BL/6J mice was provided by C57BL/6J zygote microinjection with px330-Tyr-M DNA vector and mutant ssDNA (G291T in Tyr) donor. A combination of CRISPR/Cas9 vector and optional mutant ssDNA could be expected to efficiently produce novel SNM-induced mouse models for investigating human diseases.
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