Evidence map›Paper›PMID 24879059›Full record

ArticlePloS one2014

Alternatively spliced tissue factor is not sufficient for embryonic development.

Susanna H M Sluka, Alexander Akhmedov, Johannes Vogel, Dusten Unruh, Vladimir Y Bogdanov, Giovanni G Camici, Thomas F Lüscher, Wolfram Ruf, Felix C Tanner

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Susanna H M SlukaCardiovascular Research, Institute of Physiology, University of Zurich, Zurich, Switzerland; Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland.
Alexander AkhmedovCardiovascular Research, Institute of Physiology, University of Zurich, Zurich, Switzerland; Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland.
Johannes VogelCenter for Integrative Human Physiology, University of Zurich, Zurich, Switzerland; Institute of Veterinary Physiology, University of Zurich, Zurich, Switzerland.
Dusten UnruhDivision of Hematology/Oncology, Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio, United States of America.
Vladimir Y BogdanovDivision of Hematology/Oncology, Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio, United States of America.
Giovanni G CamiciCardiovascular Research, Institute of Physiology, University of Zurich, Zurich, Switzerland; Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland.
Thomas F LüscherCardiovascular Research, Institute of Physiology, University of Zurich, Zurich, Switzerland; Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland; Cardiology, Cardiovascular Center, University Hospital Zurich, Zurich, Switzerland.
Wolfram RufDepartment of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California, United States of America.
Felix C TannerCardiovascular Research, Institute of Physiology, University of Zurich, Zurich, Switzerland; Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland; Cardiology, Cardiovascular Center, University Hospital Zurich, Zurich, Switzerland.
University of Zurich · CHUniversity of Cincinnati Medical Center · USScripps Research Institute · USSwiss Integrative Center for Human Health · CH

Funding

SIGNALING OF TISSUE FACTOR IN CELL ADHESIONR01HL060742 · NHLBI · SCRIPPS RESEARCH INSTITUTE, THE · PI RUF, WOLFRAM · 1998 to 2017
$6.4M
NHLBI NIH HHS HL-60742NHLBI NIH HHS R01 HL060742
6 · The paper itself

Abstract

Tissue factor (TF) triggers blood coagulation and is translated from two mRNA splice isoforms, encoding membrane-anchored full-length TF (flTF) and soluble alternatively-spliced TF (asTF). The complete knockout of TF in mice causes embryonic lethality associated with failure of the yolk sac vasculature. Although asTF plays roles in postnatal angiogenesis, it is unknown whether it activates coagulation sufficiently or makes previously unrecognized contributions to sustaining integrity of embryonic yolk sac vessels. Using gene knock-in into the mouse TF locus, homozygous asTF knock-in (asTFKI) mice, which express murine asTF in the absence of flTF, exhibited embryonic lethality between day 9.5 and 10.5. Day 9.5 homozygous asTFKI embryos expressed asTF protein, but no procoagulant activity was detectable in a plasma clotting assay. Although the α-smooth-muscle-actin positive mesodermal layer as well as blood islands developed similarly in day 8.5 wild-type or homozygous asTFKI embryos, erythrocytes were progressively lost from disintegrating yolk sac vessels of asTFKI embryos by day 10.5. These data show that in the absence of flTF, asTF expressed during embryonic development has no measurable procoagulant activity, does not support embryonic vessel stability by non-coagulant mechanisms, and fails to maintain a functional vasculature and embryonic survival.

Indexed as

Alternative SplicingAnimalsBlood CoagulationEmbryonic DevelopmentFemaleGene Knock-In TechniquesHomozygoteMiceNeovascularization, PhysiologicProtein Structure, TertiaryThromboplastinYolk SacThromboplastin

Identifiers

PMID24879059
PMCPMC4039448
OpenAlexW2052848538

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.