ArticlePloS one2014
Dose-response of superparamagnetic iron oxide labeling on mesenchymal stem cells chondrogenic differentiation: a multi-scale in vitro study.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Nanosensitive optical coherence tomography for detecting structural changes in stem cells.Biomedical optics express · 2023Article
- A primer onFrontiers in medicine · 2023Review
- Influence of SPIO labelling on the function of BMSCs in chemokine receptors expression and chemotaxis.PeerJ · 2023Article
- Article
- Human integrin α10β1-selected mesenchymal stem cells home to cartilage defects in the rabbit knee and assume a chondrocyte-like phenotype.Stem cell research & therapy · 2022Article
- Contrast agents for photoacoustic imaging: a review of stem cell tracking.Stem cell research & therapy · 2021Review
- Superparamagnetic Iron Oxide Particles (VSOPs) Show Genotoxic Effects but No Functional Impact on Human Adipose Tissue-Derived Stromal Cells (ASCs).Materials (Basel, Switzerland) · 2021Article
- Magnetic resonance imaging of human neural stem cells in rodent and primate brain.Stem cells translational medicine · 2021Article
- Respective stemness and chondrogenic potential of mesenchymal stem cells isolated from human bone marrow, synovial membrane, and synovial fluid.Stem cell research & therapy · 2020Article
- Combining Innovative Bioink and Low Cell Density for the Production of 3D-Bioprinted Cartilage Substitutes: A Pilot Study.Stem cells international · 2020Article
- Gadolinium-Labelled Cell Scaffolds to Follow-up Cell Transplantation by Magnetic Resonance Imaging.Journal of functional biomaterials · 2019Article
- Tracking Stem Cell Implants in Cartilage Defects of Minipigs by Using Ferumoxytol-enhanced MRI.Radiology · 2019Article
- Labeling of human mesenchymal stem cells with different classes of vital stains: robustness and toxicity.Stem cell research & therapy · 2019Article
- Experimental Strategies of Mesenchymal Stem Cell Propagation: Adverse Events and Potential Risk of Functional Changes.Stem cells international · 2019Review
- Tracking Cell Transplants in Femoral Osteonecrosis with Magnetic Resonance Imaging: A Proof-of-Concept Study in Patients.Clinical cancer research : an official journal of the American Association for Cancer Research · 2018Article
- In vitro and in vivo potentialities for cartilage repair from human advanced knee osteoarthritis synovial fluid-derived mesenchymal stem cells.Stem cell research & therapy · 2018Article
- The impact of nanoparticle-driven lysosomal alkalinization on cellular functionality.Journal of nanobiotechnology · 2018Article
- Biopolymeric In Situ Hydrogels for Tissue Engineering and Bioimaging Applications.Tissue engineering and regenerative medicine · 2018Review
- Detection of Repair of the Zone of Calcified Cartilage with Osteoarthritis through Mesenchymal Stem Cells by Ultrashort Echo Time Magnetic Resonance Imaging.Chinese medical journal · 2018Review
- Imaging Biomaterial-Tissue Interactions.Trends in biotechnology · 2018Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimThe aim of this work was the development of successful cell therapy techniques for cartilage engineering. This will depend on the ability to monitor non-invasively transplanted cells, especially mesenchymal stem cells (MSCs) that are promising candidates to regenerate damaged tissues.
methodsMSCs were labeled with superparamagnetic iron oxide particles (SPIO). We examined the effects of long-term labeling, possible toxicological consequences and the possible influence of progressive concentrations of SPIO on chondrogenic differentiation capacity.
resultsNo influence of various SPIO concentrations was noted on human bone marrow MSC viability or proliferation. We demonstrated long-term (4 weeks) in vitro retention of SPIO by human bone marrow MSCs seeded in collagenic sponges under TGF-β1 chondrogenic conditions, detectable by Magnetic Resonance Imaging (MRI) and histology. Chondrogenic differentiation was demonstrated by molecular and histological analysis of labeled and unlabeled cells. Chondrogenic gene expression (COL2A2, ACAN, SOX9, COL10, COMP) was significantly altered in a dose-dependent manner in labeled cells, as were GAG and type II collagen staining. As expected, SPIO induced a dramatic decrease of MRI T2 values of sponges at 7T and 3T, even at low concentrations.
conclusionsThis study clearly demonstrates (1) long-term in vitro MSC traceability using SPIO and MRI and (2) a deleterious dose-dependence of SPIO on TGF-β1 driven chondrogenesis in collagen sponges. Low concentrations (12.5-25 µg Fe/mL) seem the best compromise to optimize both chondrogenesis and MRI labeling.
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