Slow and sustained nitric oxide releasing compounds inhibit multipotent vascular stem cell proliferation and differentiation without causing cell death.

Brandon M Curtis, Kyle Alexander Leix, Yajing Ji, Richard Samuel Elliot Glaves, David E Ash, Dillip K Mohanty

Abstract read

In one paragraph

Article in Biochemical and biophysical research communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it

0cells of the map it votes in

7citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

Multifunctional Biodegradable Vascular PLLA Scaffold with Improved X-ray Opacity, Anti-Inflammation, and Re-Endothelization.Polymers · 2021
Article
Models for potential dendritic nitric oxide donors: crystal structures of two 2-nitroanilino precursors and nitric oxide-release behavior of the nitrosated derivatives.Acta crystallographica. Section C, Structural chemistry · 2018
Article
Oxidation Prevents HMGB1 Inhibition on PDGF-Induced Differentiation of Multipotent Vascular Stem Cells to Smooth Muscle Cells: A Possible Mechanism Linking Oxidative Stress to Atherosclerosis.BioMed research international · 2018
Article
Stem cell-derived vasculature: A potent and multidimensional technology for basic research, disease modeling, and tissue engineering.Biochemical and biophysical research communications · 2016
Review
Two N-(2-phenylethyl)nitroaniline derivatives as precursors for slow and sustained nitric oxide release agents.Acta crystallographica. Section C, Structural chemistry · 2016
Article
Nitric Oxide Modulates Postnatal Bone Marrow-Derived Mesenchymal Stem Cell Migration.Frontiers in cell and developmental biology · 2016
Article
Nitric oxide regulates multiple functions and fate of adult progenitor and stem cells.Journal of physiology and biochemistry · 2015
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Brandon M CurtisDepartment of Chemistry, Central Michigan University, Mount Pleasant, MI 48859, USA.

Kyle Alexander LeixDepartment of Chemistry, Central Michigan University, Mount Pleasant, MI 48859, USA.

Yajing JiDepartment of Biomedical Science and Medicine, Michigan State University, East Lansing, MI 48824, USA.

Richard Samuel Elliot GlavesDepartment of Biology, Central Michigan University, Mount Pleasant, MI 48859, USA.

David E AshDepartment of Chemistry, Central Michigan University, Mount Pleasant, MI 48859, USA.

Dillip K MohantyDepartment of Chemistry, Central Michigan University, Mount Pleasant, MI 48859, USA. Electronic address: Mohan1dk@cmich.edu.

Funding

Effects of Nitric Oxide on Smooth Muscle Cell ProliferationsR15HL106600 · NHLBI · CENTRAL MICHIGAN UNIVERSITY · PI MOHANTY, DILLIP · 2011 to 2011

$407k

NHLBI NIH HHS R15 HL106600NHLBI NIH HHS R15HL 106600

6 · The paper itself

Abstract

Atherosclerosis is the leading cause of cerebral and myocardial infarction. It is believed that neointimal growth common in the later stages of atherosclerosis is a result of vascular smooth muscle cell (SMC) de-differentiation in response to endothelial injury. However, the claims of the SMC de-differentiation theory have not been substantiated by monitoring the fate of mature SMCs in response to such injuries. A recent study suggests that atherosclerosis is a consequence of multipotent vascular stem cell (MVSC) differentiation. Nitric oxide (NO) is a well-known mediator against atherosclerosis, in part because of its inhibitory effect on SMC proliferation. Using three different NO-donors, we have investigated the effects of NO on MVSC proliferation. Results indicate that NO inhibits MVSC proliferation in a concentration dependent manner. A slow and sustained delivery of NO proved to inhibit proliferation without causing cell death. On the other hand, larger, single-burst NO concentrations, inhibits proliferation, with concurrent significant cell death. Furthermore, our results indicate that endogenously produced NO inhibits MVSC differentiation to mesenchymal-like stem cells (MSCs) and subsequently to SMC as well.

Indexed as

AnimalsApoptosisCell DifferentiationCell ProliferationCells, CulturedDelayed-Action PreparationsMesenchymal Stem CellsMultipotent Stem CellsMyocytes, Smooth MuscleNitric Oxide DonorsOxidative StressRatsDelayed-Action PreparationsNitric Oxide DonorsMesenchymal-like stem cellsMultipotent vascular stem cellsSlow and sustained nitric oxide donorSmooth muscle cellsStem cell proliferation and differentiation

Identifiers

PMID24878532

DOI10.1016/j.bbrc.2014.05.087

PMCPMC4107195

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.