Evidence map›Paper›PMID 24878526›Full record

ArticleBiochemical and biophysical research communications2014

Conservation of sequence and function in fertilization of the cortical granule serine protease in echinoderms.

Nathalie Oulhen, Dongdong Xu, Gary M Wessel

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical and biophysical research communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 91% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 2 countries.

Nathalie OulhenDepartment of Molecular and Cell Biology and Biochemistry, Brown University, 185 Meeting St., Providence, RI 02912, USA.
Dongdong XuDepartment of Molecular and Cell Biology and Biochemistry, Brown University, 185 Meeting St., Providence, RI 02912, USA; Marine Fishery Institute of Zhejiang Province, Key Lab of Mariculture and Enhancement of Zhejiang Province, 316100 Zhoushan, Zhejiang Province, PR China.
Gary M WesselDepartment of Molecular and Cell Biology and Biochemistry, Brown University, 185 Meeting St., Providence, RI 02912, USA. Electronic address: rhet@brown.edu.
Brown University · US

Funding

Post-translational Regulation of Germline DeterminantsR01HD028152 · NICHD · BROWN UNIVERSITY · PI WESSEL, GARY M · 1997 to 2016
$3.9M
Shared High-throughput DNA sequencer for the Brown University CommunityS10RR027634 · NCRR · BROWN UNIVERSITY · PI WESSEL, GARY M · 2010 to 2010
$500k
ONTOGENY OF THE SEA URCHIN ENDODERM LINEAGER29HD028152 · NICHD · BROWN UNIVERSITY · PI WESSEL, GARY M · 1991 to 1995
–
NCRR NIH HHS S10 RR027634NICHD NIH HHS HD028152NICHD NIH HHS R01 HD028152
6 · The paper itself

Abstract

Conservation of the cortical granule serine protease during fertilization in echinoderms was tested both functionally in sea stars, and computationally throughout the echinoderm phylum. We find that the inhibitor of serine protease (soybean trypsin inhibitor) effectively blocks proper transition of the sea star fertilization envelope into a protective sperm repellent, whereas inhibitors of the other main types of proteases had no effect. Scanning the transcriptomes of 15 different echinoderm ovaries revealed sequences of high conservation to the originally identified sea urchin cortical serine protease, CGSP1. These conserved sequences contained the catalytic triad necessary for enzymatic activity, and the tandemly repeated LDLr-like repeats. We conclude that the protease involved in the slow block to polyspermy is an essential and conserved element of fertilization in echinoderms, and may provide an important reagent for identification and testing of the cell surface proteins in eggs necessary for sperm binding.

Indexed as

Amino Acid SequenceAnimalsConserved SequenceEchinodermataFemaleFertilizationMaleMolecular Sequence DataOvumPhylogenySequence Homology, Amino AcidSerine ProteasesSerine Proteinase InhibitorsSperm-Ovum InteractionsStrongylocentrotus purpuratusTranscriptomeSerine ProteasesSerine Proteinase InhibitorsCortical granule proteaseCortical granulesEchinodermsEgg receptor for spermFertilizationSea star

Identifiers

PMID24878526
PMCPMC4133295
OpenAlexW2055748435

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.