ArticleMolecular & cellular proteomics : MCP2014
Highly precise quantification of protein molecules per cell during stress and starvation responses in Bacillus subtilis.
Article in Molecular & cellular proteomics : MCP, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 51 citations in OpenAlex.
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- The nucleotide messenger (p)ppGpp is an anti-inducer of the purine synthesis transcription regulator PurR in Bacillus.Nucleic acids research · 2022Article
- Moonlighting inMicroorganisms · 2021Review
- Proteomic Adaptation ofCells · 2021Article
- Advances on systems metabolic engineering ofSynthetic and systems biotechnology · 2020Article
- Molecular Mechanism of Regulation of the Purine Salvage Enzyme XPRT by the Alarmones pppGpp, ppGpp, and pGpp.Journal of molecular biology · 2020Article
- The alarmones (p)ppGpp are part of the heat shock response of Bacillus subtilis.PLoS genetics · 2020Article
- Membrane Modulation of Super-Secreting "midiFrontiers in bioengineering and biotechnology · 2020Article
- Proteomic Signatures ofCells · 2018Article
- Application of targeted mass spectrometry in bottom-up proteomics for systems biology research.Journal of proteomics · 2018Review
- Proteomic Analysis of Normal Expression Differences Exist in Bacillus Subtilis 168 Cultivation.Current microbiology · 2018Article
- Structural basis for the regulatory interaction of the methylglyoxal synthase MgsA with the carbon flux regulator Crh inThe Journal of biological chemistry · 2018Article
- LC-MS/MS proteomic analysis of starved Bacillus subtilis cells overexpressing ribonucleotide reductase (nrdEF): implications in stress-associated mutagenesis.Current genetics · 2018Article
- Structure of the Branched-chain Amino Acid and GTP-sensing Global Regulator, CodY, fromThe Journal of biological chemistry · 2017Article
- The Blueprint of a Minimal Cell: MiniBacillus.Microbiology and molecular biology reviews : MMBR · 2016Review
- Stress Physiology of Lactic Acid Bacteria.Microbiology and molecular biology reviews : MMBR · 2016Review
- Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.Scientific reports · 2016Article
- SubtiWiki 2.0--an integrated database for the model organism Bacillus subtilis.Nucleic acids research · 2016Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Systems biology based on high quality absolute quantification data, which are mandatory for the simulation of biological processes, successively becomes important for life sciences. We provide protein concentrations on the level of molecules per cell for more than 700 cytosolic proteins of the Gram-positive model bacterium Bacillus subtilis during adaptation to changing growth conditions. As glucose starvation and heat stress are typical challenges in B. subtilis' natural environment and induce both, specific and general stress and starvation proteins, these conditions were selected as models for starvation and stress responses. Analyzing samples from numerous time points along the bacterial growth curve yielded reliable and physiologically relevant data suitable for modeling of cellular regulation under altered growth conditions. The analysis of the adaptational processes based on protein molecules per cell revealed stress-specific modulation of general adaptive responses in terms of protein amount and proteome composition. Furthermore, analysis of protein repartition during glucose starvation showed that biomass seems to be redistributed from proteins involved in amino acid biosynthesis to enzymes of the central carbon metabolism. In contrast, during heat stress most resources of the cell, namely those from amino acid synthetic pathways, are used to increase the amount of chaperones and proteases. Analysis of dynamical aspects of protein synthesis during heat stress adaptation revealed, that these proteins make up almost 30% of the protein mass accumulated during early phases of this stress.
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