ArticleMolecular and biochemical parasitology2014
A dual luciferase system for analysis of post-transcriptional regulation of gene expression in Leishmania.
Article in Molecular and biochemical parasitology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed.
- The necroptosis-related lncRNA ENSG00000253385.1 promotes the progression of esophageal squamous cell carcinoma by targeting the miR-16-2-3p/VDAC1 axis.Scientific reports · 2025Article
- Reporter gene systems: A powerful tool forCurrent research in microbial sciences · 2022Article
- Purine-responsive expression of theThe Journal of biological chemistry · 2020Article
- Dynamic regulation of the Trypanosoma brucei transferrin receptor in response to iron starvation is mediated via the 3'UTR.PloS one · 2018Article
- Integrating ribosomal promoter vectors that offer a choice of constitutive expression profiles in Leishmania donovani.Molecular and biochemical parasitology · 2015Article
- The past, present, and future of Leishmania genomics and transcriptomics.Trends in parasitology · 2015Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Gene expression in kinetoplastid parasites is regulated via post-transcriptional mechanisms that modulate mRNA turnover, translation rate, and/or post-translational protein stability. To facilitate the analysis of post-transcriptional regulation, a dual luciferase system was developed in which firefly and Renilla luciferase reporters genetically fused to compatible drug resistance genes are integrated in place of one allele of the gene of interest and of an internal control gene, respectively, in a manner that preserves the cognate pre-mRNA processing signals. The sensitivity and reproducibility of the assay coupled with the ability to rapidly assemble reporter integration constructs render the dual luciferase system suitable for analysis of multiple candidates derived from global expression analysis platforms. To demonstrate the utility of the system, regulation of three genes in response to purine starvation was examined in Leishmania donovani promastigotes. This dual luciferase system should be directly applicable to the analysis of post-transcriptional regulation in other kinetoplastids.
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Registered trials
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