ArticleBioMed research international2014
Complete cell killing by applying high hydrostatic pressure for acellular vascular graft preparation.
Article in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Evaluation of Apoptotic Caspase-3 Immunopositivity in Human Model of Asphyxial Death.International journal of molecular sciences · 2025Article
- Effect of high cyclic hydrostatic pressure on osteogenesis of mesenchymal stem cells cultured in liquefied micro-compartments.Materials today. Bio · 2023Article
- Establishing safe high hydrostatic pressure devitalization thresholds for autologous head and neck cancer vaccination and reconstruction.Cell death discovery · 2023Article
- Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein Preservation.Bioengineering (Basel, Switzerland) · 2023Article
- Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues.International journal of molecular sciences · 2022Article
- Research and application of hydrostatic high pressure in tumor vaccines (Review).Oncology reports · 2021Review
- Long-term observation of airway reconstruction using decellularized tracheal allografts in micro-miniature pigs at growing stage.Regenerative therapy · 2020Article
- Extracorporeal high-pressure therapy (EHPT) for malignant melanoma consisting of simultaneous tumor eradication and autologous dermal substitute preparation.Regenerative therapy · 2020Article
- Fundamental Study of Decellularization Method Using Cyclic Application of High Hydrostatic Pressure.Micromachines · 2020Article
- Hydrostatic pressure can induce apoptosis of the skin.Scientific reports · 2020Article
- Simple and efficient method for consecutive inactivation-cryopreservation of porcine skin grafts.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2020Article
- High Hydrostatic Pressure Therapy Annihilates Squamous Cell Carcinoma in a Murine Model.BioMed research international · 2020Article
- Exploration of the Pressurization Condition for Killing Human Skin Cells and Skin Tumor Cells by High Hydrostatic Pressure.BioMed research international · 2020Article
- Article
- Melanin pigments in the melanocytic nevus regress spontaneously after inactivation by high hydrostatic pressure.PloS one · 2017Article
- The Alteration of the Epidermal Basement Membrane Complex of Human Nevus Tissue and Keratinocyte Attachment after High Hydrostatic Pressurization.BioMed research international · 2016Article
- Preparation of Inactivated Human Skin Using High Hydrostatic Pressurization for Full-Thickness Skin Reconstruction.PloS one · 2015Article
- The rapid inactivation of porcine skin by applying high hydrostatic pressure without damaging the extracellular matrix.BioMed research international · 2015Article
- Application of bladder acellular matrix in urinary bladder regeneration: the state of the art and future directions.BioMed research international · 2015Review
- Comprehensive characterization of cell and tissue responses toward high hydrostatic pressure treatment: Molecular feedback and structural integrity in bone graft processing.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pressure treatment has been developed in tissue engineering application. Although the tissue scaffold prepared by a ultrahydrostatic pressure treatment has been reported, an excessive pressure has a potential to disrupt a structure of extracellular matrix through protein denaturation. It is important to understand the suitable low-pressure condition and mechanisms for cell killing. In this study, cellular morphology, mitochondria activity, and membrane permeability of mammalian cells with various pressure treatments were investigated with in vitro models. When the cells were treated with a pressure of 100 MPa for 10 min, cell morphology and adherence were the same as an untreated cells. Dehydrogenase activity in mitochondria was almost the same as untreated cells. On the other hand, when the cells were treated with the pressure of more than 200 MPa, the cells did not adhere, and the dehydrogenase activity was completely suppressed. However, green fluorescence was observed in the live/dead staining images, and the cells were completely stained as red after above 500 MPa. That is, membrane permeability was disturbed with the pressure treatment of above 500 MPa. These results indicated that the pressure of 200 MPa for 10 min was enough to induce cell killing through inactivation of mitochondria activity.
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