ArticleBiomedical optics express2014
Confocal Raman micro-spectroscopy for rapid and label-free detection of maleic acid-induced variations in human sperm.
Article in Biomedical optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Melatonin Supplementation in Sex-Sorted Nili-Ravi Buffalo Semen: Effect on Sperm Quality, Subsequent in Vitro Embryo Development, and Pregnancy Outcomes.Antioxidants (Basel, Switzerland) · 2026Article
- Predicting Boar Sperm Survival during Liquid Storage Using Vibrational Spectroscopic Techniques.Biology · 2024Article
- Decoding the role of cytochrome c in metabolism of human spermatozoa by Raman imaging.Frontiers in cell and developmental biology · 2022Article
- Label-Free Evaluation of Chromatin Condensation in Human Normal Morphology Sperm Using Raman Spectroscopy.Reproductive sciences (Thousand Oaks, Calif.) · 2021Article
- Application and Progress of Raman Spectroscopy in Male Reproductive System.Frontiers in cell and developmental biology · 2021Review
- Sperm quality of rats exposed to difenoconazole using classical parameters and surface-enhanced Raman scattering: classification performance by machine learning methods.Environmental science and pollution research international · 2019Article
- Combined Raman and polarization sensitive holographic imaging for a multimodal label-free assessment of human sperm function.Scientific reports · 2019Article
- Advances in Cryopreservation of Bull Sperm.Frontiers in veterinary science · 2019Review
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Authors and funding
5 authors.
Funding
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Abstract
Confocal Raman microspectroscopy is a valuable analytical tool in biological and medical research, allowing the detection of sample variations without external labels or extensive preparation. To determine whether this method can assess the effect of maleic acid on sperm, we prepared human sperm samples incubated in different concentrations of maleic acid, after which Raman spectra from the various regions of sperm cells were recorded. Following the maleic acid treatment, Raman spectra indicated significant changes. Combined with other means, we found that the structures and chemical compositions of sperm membranes were damaged, and even the sperm DNA was damaged by the incorporation of maleic acid. Thus, this technique can be used for detection and identification of maleic acid-induced changes in human sperm at a molecular level. Although this particular application of Raman microspectroscopy still requires further validation, it has potentially promise as a diagnostic tool for reproductive medicine.
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