ArticleBiomedical optics express2014
Cellular imaging of deep organ using two-photon Bessel light-sheet nonlinear structured illumination microscopy.
Article in Biomedical optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 75 citations in OpenAlex.
- Two-photon light-sheet microscopy with isotropic near-diffraction-limited resolution in three dimensions.iScience · 2026Article
- Two-photon 3D imaging of optically stimulated neural activity at 100 Hz.Light, science & applications · 2026Article
- Rapid axially scanned and de-scanned line-scan confocal microscopy with a tunable acoustic gradient index of refraction lens for high-speed volumetricNeurophotonics · 2025Article
- Frontiers in artificial intelligence-directed light-sheet microscopy for uncovering biological phenomena and multi-organ imaging.View (Beijing, China) · 2024Article
- Super-Resolution Imaging Reveals the Nanoscale Distributions of Dystroglycan and Integrin Itga7 in Zebrafish Muscle Fibers.Biomedicines · 2023Article
- Extended Depth of Focus Two-Photon Light-Sheet Microscopy for In Vivo Fluorescence Imaging of Large Multicellular Organisms at Cellular Resolution.International journal of molecular sciences · 2023Article
- Depth random-access two-photon Bessel light-sheet imaging in brain tissue.Optics express · 2022Article
- Fast whole-brain imaging of seizures in zebrafish larvae by two-photon light-sheet microscopy.Biomedical optics express · 2022Article
- Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination.Nature communications · 2021Article
- In vivo NIR-II structured-illumination light-sheet microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Advances in fluorescence microscopy techniques to study kidney function.Nature reviews. Nephrology · 2021Review
- To be more precise: the role of intracellular trafficking in development and pattern formation.Biochemical Society transactions · 2020Review
- Structured illumination imaging with quasi-periodic patterns.Journal of biophotonics · 2020Article
- Propagating-path uniformly scanned light sheet excitation microscopy for isotropic volumetric imaging of large specimens.Journal of biomedical optics · 2019Article
- Imaging neural events in zebrafish larvae with linear structured illumination light sheet fluorescence microscopy.Neurophotonics · 2019Article
- Depth-extended, high-resolution fluorescence microscopy: whole-cell imaging with double-ring phase (DRiP) modulation.Biomedical optics express · 2019Article
- Fast 3-D Imaging of Brain Organoids With a New Single-Objective Planar-Illumination Two-Photon Microscope.Frontiers in neuroanatomy · 2019Article
- A Guide to Emerging Technologies for Large-Scale and Whole-Brain Optical Imaging of Neuronal Activity.Annual review of neuroscience · 2018Review
- Light sheet approaches for improved precision in 3D localization-based super-resolution imaging in mammalian cells [Invited].Optics express · 2018Review
- Novel Microscopic Techniques for Podocyte Research.Frontiers in endocrinology · 2018Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
In vivo fluorescent cellular imaging of deep internal organs is highly challenging, because the excitation needs to penetrate through strong scattering tissue and the emission signal is degraded significantly by photon diffusion induced by tissue-scattering. We report that by combining two-photon Bessel light-sheet microscopy with nonlinear structured illumination microscopy (SIM), live samples up to 600 microns wide can be imaged by light-sheet microscopy with 500 microns penetration depth, and diffused background in deep tissue light-sheet imaging can be reduced to obtain clear images at cellular resolution in depth beyond 200 microns. We demonstrate in vivo two-color imaging of pronephric glomeruli and vasculature of zebrafish kidney, whose cellular structures located at the center of the fish body are revealed in high clarity by two-color two-photon Bessel light-sheet SIM.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.