ArticleBiology open2014
Inward cholesterol gradient of the membrane system in P. falciparum-infected erythrocytes involves a dilution effect from parasite-produced lipids.
Article in Biology open, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Pivotal roles of Plasmodium falciparum lysophospholipid acyltransferase 1 in cell cycle progression and cytostome internalization.Communications biology · 2025Article
- Harnessing cholesterol uptake of malaria parasites for therapeutic applications.EMBO molecular medicine · 2024Article
- Role of TAM Receptors in Antimalarial Humoral Immune Response.Pathogens (Basel, Switzerland) · 2024Review
- The enemy within: lipid asymmetry in intracellular parasite-host interactions.Emerging topics in life sciences · 2023Review
- Automated Recognition of Plasmodium falciparum Parasites from Portable Blood Levitation Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Dramatic Consequences of Reducing Erythrocyte Membrane Cholesterol on Plasmodium falciparum.Microbiology spectrum · 2022Article
- The role of cholesterol in invasion and growth of malaria parasites.Frontiers in cellular and infection microbiology · 2022Review
- Transport mechanisms at the malaria parasite-host cell interface.PLoS pathogens · 2021Review
- Breakdown in membrane asymmetry regulation leads to monocyte recognition of P. falciparum-infected red blood cells.PLoS pathogens · 2021Article
- Malaria Parasites Hijack Host Receptors From Exosomes to Capture Lipoproteins.Frontiers in cell and developmental biology · 2021Article
- A Profound Membrane Reorganization Defines Susceptibility ofFrontiers in immunology · 2021Article
- Characterization of the Tubovesicular Network inFrontiers in cellular and infection microbiology · 2021Article
- Mechanism of action of glycyrrhizin against Plasmodium falciparum.Memorias do Instituto Oswaldo Cruz · 2021Article
- Depletion of cholesterol could be associated with modulation of progesterone but not other sex hormone levels during Plasmodium falciparum infection in humans: a cross-sectional study from Zaria, Nigeria.Parasitology research · 2020Article
- γδ T Cells KillJournal of immunology (Baltimore, Md. : 1950) · 2020Article
- Cholesterol-dependent enrichment of understudied erythrocytic stages of human Plasmodium parasites.Scientific reports · 2020Article
- Real-time cholesterol sorting in Plasmodium falciparum-erythrocytes as revealed by 3D label-free imaging.Scientific reports · 2020Article
- Hardly Vacuous: The Parasitophorous Vacuolar Membrane of Malaria Parasites.Trends in parasitology · 2020Review
- Polyunsaturated fatty acids promoteBiology open · 2019Article
- Diverse Chemical Compounds Target Plasmodium falciparum Plasma Membrane Lipid Homeostasis.ACS infectious diseases · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Plasmodium falciparum (Pf) infection remodels the human erythrocyte with new membrane systems, including a modified host erythrocyte membrane (EM), a parasitophorous vacuole membrane (PVM), a tubulovesicular network (TVN), and Maurer's clefts (MC). Here we report on the relative cholesterol contents of these membranes in parasitized normal (HbAA) and hemoglobin S-containing (HbAS, HbAS) erythrocytes. Results from fluorescence lifetime imaging microscopy (FLIM) experiments with a cholesterol-sensitive fluorophore show that membrane cholesterol levels in parasitized erythrocytes (pRBC) decrease inwardly from the EM, to the MC/TVN, to the PVM, and finally to the parasite membrane (PM). Cholesterol depletion of pRBC by methyl-β-cyclodextrin treatment caused a collapse of this gradient. Lipid and cholesterol exchange data suggest that the cholesterol gradient involves a dilution effect from non-sterol lipids produced by the parasite. FLIM signals from the PVM or PM showed little or no difference between parasitized HbAA vs HbS-containing erythrocytes that differed in lipid content, suggesting that malaria parasites may regulate the cholesterol contents of the PVM and PM independently of levels in the host cell membrane. Cholesterol levels may affect raft structures and the membrane trafficking and sorting functions that support Pf survival in HbAA, HbAS and HbSS erythrocytes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.