ArticleNucleic acids research2014
The strength of the template effect attracting nucleotides to naked DNA.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 57 citations in OpenAlex.
- Yield and fidelity of early steps of ribosome-free translation depend on the RNA anchoring.Nucleic acids research · 2026Article
- A quantitative model of enzyme-free copying of RNA with dimers.Nucleic acids research · 2025Article
- Sequencing of d/l-DNA and XNA by Templated-Synthesis.Journal of the American Chemical Society · 2025Article
- The RNA-DNA world and the emergence of DNA-encoded heritable traits.RNA biology · 2024Article
- Insight into the structures of unusual base pairs in RNA complexes containing a primer/template/adenosine ligand.RSC chemical biology · 2023Article
- Thermodynamics and kinetics of DNA and RNA dinucleotide hybridization to gaps and overhangs.Biophysical journal · 2023Article
- Direct monitoring of the thermodynamics and kinetics of DNA and RNA dinucleotide dehybridization from gaps and overhangs.bioRxiv : the preprint server for biology · 2023Article
- Synthesis of a Peptidoyl RNA Hairpin via a Combination of Solid-Phase and Template-Directed Chain Assembly.Chembiochem : a European journal of chemical biology · 2022Article
- Enzyme-Free Copying of 12 Bases of RNA with Dinucleotides.Angewandte Chemie (International ed. in English) · 2022Article
- High Fidelity Enzyme-Free Primer Extension with an Ethynylpyridone Thymidine Analog.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Structure-Activity Relationships in Nonenzymatic Template-Directed RNA Synthesis.Angewandte Chemie (International ed. in English) · 2021Article
- 2'/3' Regioselectivity of Enzyme-Free Copying of RNA Detected by NMR.Chembiochem : a European journal of chemical biology · 2020Article
- Enzyme-free ligation of dimers and trimers to RNA primers.Nucleic acids research · 2019Article
- Structural Rationale for the Enhanced Catalysis of Nonenzymatic RNA Primer Extension by a Downstream Oligonucleotide.Journal of the American Chemical Society · 2018Article
- Enzyme-free genetic copying of DNA and RNA sequences.Beilstein journal of organic chemistry · 2018Review
- A Kinetic Model of Nonenzymatic RNA Polymerization by Cytidine-5'-phosphoro-2-aminoimidazolide.Biochemistry · 2017Article
- Insight into the mechanism of nonenzymatic RNA primer extension from the structure of an RNA-GpppG complex.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Downstream Oligonucleotides Strongly Enhance the Affinity of GMP to RNA Primer-Template Complexes.Journal of the American Chemical Society · 2017Article
- Unusual Hydrogen Bonding Patterns and the Role of the Backbone in Nucleic Acid Information Transfer.ACS central science · 2016Article
- Unusual Base-Pairing Interactions in Monomer-Template Complexes.ACS central science · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transmission of genetic information relies on Watson-Crick base pairing between nucleoside phosphates and template bases in template-primer complexes. Enzyme-free primer extension is the purest form of the transmission process, without any chaperon-like effect of polymerases. This simple form of copying of sequences is intimately linked to the origin of life and provides new opportunities for reading genetic information. Here, we report the dissociation constants for complexes between (deoxy)nucleotides and template-primer complexes, as determined by nuclear magnetic resonance and the inhibitory effect of unactivated nucleotides on enzyme-free primer extension. Depending on the sequence context, Kd's range from 280 mM for thymidine monophosphate binding to a terminal adenine of a hairpin to 2 mM for a deoxyguanosine monophosphate binding in the interior of a sequence with a neighboring strand. Combined with rate constants for the chemical step of extension and hydrolytic inactivation, our quantitative theory explains why some enzyme-free copying reactions are incomplete while others are not. For example, for GMP binding to ribonucleic acid, inhibition is a significant factor in low-yielding reactions, whereas for amino-terminal DNA hydrolysis of monomers is critical. Our results thus provide a quantitative basis for enzyme-free copying.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.