ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2014
Concordance between RT-PCR-based detection of respiratory viruses from nasal swabs collected for viral testing and nasopharyngeal swabs collected for bacterial testing.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- Article
- Specimen adequacy assay controls in nucleic acid amplification tests do not correlate with nasopharyngeal swab collection method.Journal of clinical microbiology · 2024Article
- Co-occurrence of bacteria and viruses and serotype distribution ofFrontiers in public health · 2024Article
- Article
- Concordance in RT-PCR detection of SARS-CoV-2 between samples preserved in viral and bacterial transport medium.Journal of virological methods · 2022Article
- Respiratory virus detection in the upper respiratory tract of asymptomatic, community-dwelling older people.BMC infectious diseases · 2022Article
- Exposure to bacterial and fungal bioaerosols in facilities processing biodegradable waste.Frontiers in public health · 2022Article
- Viral Coinfection and Nasal Cytokines in Children With Clinically Diagnosed Acute Sinusitis.Frontiers in pediatrics · 2021Article
- Suspension microarray-based comparison of oropharyngeal swab and bronchoalveolar lavage fluid for pathogen identification in young children hospitalized with respiratory tract infection.BMC infectious diseases · 2020Article
- Respiratory syncytial, parainfluenza and influenza virus infection in young children with acute lower respiratory infection in rural Gambia.Scientific reports · 2019Article
- Nasal or throat sampling is adequate for the detection of the human respiratory syncytial virus in children with acute respiratory infections.Journal of medical virology · 2019Article
- Clinical Practice Guidelines by the Infectious Diseases Society of America: 2018 Update on Diagnosis, Treatment, Chemoprophylaxis, and Institutional Outbreak Management of Seasonal Influenzaa.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2019Article
- A novel real-time RT-PCR assay for influenza C tested in Peruvian children.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2017Article
- Update on Human Rhinovirus and Coronavirus Infections.Seminars in respiratory and critical care medicine · 2016Review
- Molecular Epidemiology of Rhinovirus Detections in Young Children.Open forum infectious diseases · 2016Article
- Article
- Respiratory Viral Detections During Symptomatic and Asymptomatic Periods in Young Andean Children.The Pediatric infectious disease journal · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
backgroundEpidemiologic studies of respiratory infections frequently rely on separate sample collections for the detection of bacteria and viruses. The requirement for two specimens presents cost, logistical, and acceptability challenges.
objectivesTo determine the agreement in detection of respiratory viruses using RT-PCR between two different types of samples collected on the same day: nasal swabs preserved in viral transport medium (NS) and nasopharyngeal swabs preserved in skim milk-tryptone-glucose-glycerol [STGG] medium (NP), the current standard for pneumococcal colonization studies. STUDY
designPaired NS and NP samples were collected between May 2009 and September 2011 as part of the RESPIRA-PERU study, a large prospective cohort of Andean children <3 years of age. NS samples used polyester swabs and viral transport medium whereas NP samples used rayon wire-handled swabs and STGG medium. Samples were tested for influenza, human metapneumovirus (MPV), respiratory syncytial virus (RSV), human rhinovirus (HRV), parainfluenza virus 3 (PIV3) and adenovirus (ADV) using real-time RT-PCR. We calculated the agreement, and compared cycle thresholds (CT) between NP and NS samples.
resultsAmong 226 paired NP-NS samples, we observed very high agreement with a Kappa statistic ranging from 0.71 for ADV to 0.97 for MPV. CT values were similar for both strategies.
conclusionsNP samples preserved in STGG provide a simple and reliable strategy for identification of both pneumococcus and respiratory viruses. This single specimen collection strategy could be used for epidemiologic studies, especially in resource-limited settings. Furthermore, archived NP-STGG specimens from previous studies could be reliably tested by RT-PCR for viruses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.