ArticleScientific reports2014
A viral over-expression system for the major malaria mosquito Anopheles gambiae.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Infectious transgenesis in arthropods.bioRxiv : the preprint server for biology · 2026Article
- Article
- Expression of endogenous Anopheles gambiae microRNAs using an Anopheles gambiae densovirus (AgDNV) intronic expression system.Parasites & vectors · 2025Article
- Sexual transmission of Anopheles gambiae densovirus (AgDNV) leads to disseminated infection in mated females.Parasites & vectors · 2022Article
- Negeviruses Reduce Replication of Alphaviruses during Coinfection.Journal of virology · 2021Article
- Mosquito Trilogy: Microbiota, Immunity and Pathogens, and Their Implications for the Control of Disease Transmission.Frontiers in microbiology · 2021Review
- Baculovirus as an efficient vector for gene delivery into mosquitoes.Scientific reports · 2018Article
- Densonucleosis viruses ('densoviruses') for mosquito and pathogen control.Current opinion in insect science · 2018Review
- Isolation and identification of Asaia sp. in Anopheles spp. mosquitoes collected from Iranian malaria settings: steps toward applying paratransgenic tools against malaria.Parasites & vectors · 2018Article
- Structure and transcription of the Helicoverpa armigera densovirus (HaDV2) genome and its expression strategy in LD652 cells.Virology journal · 2017Article
- Microbial control of arthropod-borne disease.Memorias do Instituto Oswaldo Cruz · 2017Review
- Viral Delivery of dsRNA for Control of Insect Agricultural Pests and Vectors of Human Disease: Prospects and Challenges.Frontiers in physiology · 2017Review
- Mosquitoes boost body armor to resist insecticide attack.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Article
- Diverse Array of New Viral Sequences Identified in Worldwide Populations of the Asian Citrus Psyllid (Diaphorina citri) Using Viral Metagenomics.Journal of virology · 2015Article
- The microbiome modulates arbovirus transmission in mosquitoes.Current opinion in virology · 2015Review
- In vitro and in vivo host range of Anopheles gambiae densovirus (AgDNV).Scientific reports · 2015Article
- Fighting Arbovirus Transmission: Natural and Engineered Control of Vector Competence in Aedes Mosquitoes.Insects · 2015Review
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Understanding pathogen/mosquito interactions is essential for developing novel strategies to control mosquito-borne diseases. Technical advances in reverse-genetics, such as RNA interference (RNAi), have facilitated elucidation of components of the mosquito immune system that are antagonistic to pathogen development, and host proteins essential for parasite development. Forward genetic approaches, however, are limited to generation of transgenic insects, and while powerful, mosquito transgenesis is a resource- and time-intensive technique that is not broadly available to most laboratories. The ability to easily "over-express" genes would enhance molecular studies in vector biology and expedite elucidation of pathogen-refractory genes without the need to make transgenic insects. We developed and characterized an efficient Anopheles gambiae densovirus (AgDNV) over-expression system for the major malaria vector Anopheles gambiae. High-levels of gene expression were detected at 3 days post-infection and increased over time, suggesting this is an effective system for gene induction. Strong expression was observed in the fat body and ovaries. We validated multiple short promoters for gene induction studies. Finally, we developed a polycistronic system to simultaneously express multiple genes of interest. This AgDNV-based toolset allows for consistent transduction of genes of interest and will be a powerful molecular tool for research in Anopheles gambiae mosquitoes.
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