ArticlePloS one2014
Deficient production of reactive oxygen species leads to severe chronic DSS-induced colitis in Ncf1/p47phox-mutant mice.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- NADPH oxidases in Parkinson's disease: a systematic review.Molecular neurodegeneration · 2017Pooled it
- Article
- Mild Chronic Colitis Exacerbates Intracerebral Inflammation in Mice with Parkinson's Disease Through LRRK2-Mediated Regulation of NF-κB Activation and Inhibition of Nrf2.Journal of inflammation research · 2025Article
- Preventive Effect ofMolecules (Basel, Switzerland) · 2023Article
- Mutant LRRK2 exacerbates immune response and neurodegeneration in a chronic model of experimental colitis.Acta neuropathologica · 2023Article
- Is LRRK2 the missing link between inflammatory bowel disease and Parkinson's disease?NPJ Parkinson's disease · 2021Review
- From Flies to Men: ROS and the NADPH Oxidase in Phagocytes.Frontiers in cell and developmental biology · 2021Review
- Oral administration of Korean propolis extract ameliorates DSS-induced colitis in BALB/c mice.International journal of medical sciences · 2020Article
- Early life nutrition influences susceptibility to chronic inflammatory colitis in later life.Scientific reports · 2019Article
- Inherited p40phox deficiency differs from classic chronic granulomatous disease.The Journal of clinical investigation · 2018Article
- NADPH oxidases and ROS signaling in the gastrointestinal tract.Mucosal immunology · 2018Review
- Reactive Oxygen Species Deficiency Due to Ncf1-Mutation Leads to Development of Adenocarcinoma and Metabolomic and Lipidomic Remodeling in a New Mouse Model of Dextran Sulfate Sodium-Induced Colitis.Frontiers in immunology · 2018Article
- ROS in gastrointestinal inflammation: Rescue Or Sabotage?British journal of pharmacology · 2017Review
- Azacytidine Treatment Inhibits the Progression of Herpes Stromal Keratitis by Enhancing Regulatory T Cell Function.Journal of virology · 2017Article
- Defective IL-23/IL-17 Axis Protects p47phox-/- Mice from Colon Cancer.Frontiers in immunology · 2017Article
- Treatment of CGD-associated Colitis with the IL-23 Blocker Ustekinumab.Journal of clinical immunology · 2016Article
- Article
- Neutrophils in animal models of autoimmune disease.Seminars in immunology · 2016Review
- Making sense of the cause of Crohn's - a new look at an old disease.F1000Research · 2016Review
- Genetic disorders coupled to ROS deficiency.Redox biology · 2015Review
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundColitis is a common clinical complication in chronic granulomatous disease (CGD), a primary immunodeficiency caused by impaired oxidative burst. Existing experimental data from NADPH-oxidase knockout mice propose contradictory roles for the involvement of reactive oxygen species in colitis chronicity and severity. Since genetically controlled mice with a point-mutation in the Ncf1 gene are susceptible to chronic inflammation and autoimmunity, we tested whether they presented increased predisposition to develop chronic colitis.
methodsColitis was induced in Ncf1-mutant and wild-type mice by a 1st 7-days cycle of dextran sulfate sodium (DSS), intercalated by a 7-days resting period followed by a 2nd 7-days DSS-cycle. Cytokines were quantified locally in the colon inflammatory infiltrates and in the serum. Leukocyte infiltration and morphological alterations of the colon mucosa were assessed by immunohistochemistry.
resultsClinical scores demonstrated a more severe colitis in Ncf1-mutant mice than controls, with no recovery during the resting period and a severe chronic colitis after the 2nd cycle, confirmed by histopathology and presence of infiltrating neutrophils, macrophages, plasmocytes and lymphocytes in the colon. Severe colitis was mediated by increased local expression of cytokines (IL-6, IL-10, TNF-α, IFN-γ and IL-17A) and phosphorylation of Leucine-rich repeat kinase 2 (LRRK2). Serological cytokine titers of those inflammatory cytokines were more elevated in Ncf1-mutant than control mice, and were accompanied by systemic changes in functional subsets of monocytes, CD4+ T and B cells.
conclusionThis suggests that an ineffective oxidative burst leads to severe chronic colitis through local accumulation of peroxynitrites, pro-inflammatory cytokines and lymphocytes and systemic immune deregulation similar to CGD.
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