ArticleNature communications2014
Generation of organized germ layers from a single mouse embryonic stem cell.
Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
What it found
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Who cites it
69 citing papers in PubMed, 124 citations in OpenAlex.
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- Acoustic cell patterning reveals geometry- and substrate-dependent vasculogenesis and human embryo model development.Nature communications · 2026Article
- Cardio-Vascular Extracellular Matrix: The Unmet Enigma.International journal of molecular sciences · 2026Review
- Three-dimensional traction technology and its application in mechanomedicine.Mechanobiology in medicine · 2025Review
- Three-Dimensional Trilineage Differentiation Conditions for Human Induced Pluripotent Stem Cells.Bioengineering (Basel, Switzerland) · 2025Article
- Nuclease-Resistant L-DNA Tension Probes Enable Long-Term Force Mapping of Single Cells and Cell Consortia.Angewandte Chemie (International ed. in English) · 2024Article
- Rotating culture regulates the formation of HepaRG-derived liver organoids via YAP translocation.BMC biology · 2024Article
- Loss of Cell-Cell Contact Inhibits Cellular Differentiation of α-Catenin Knock Out P19 Embryonal Carcinoma Cells and Their Colonization into the Developing Mouse Embryos.Biotech (Basel (Switzerland)) · 2024Article
- Orai1 and Orai3 act through distinct signalling axes to promote stemness and tumorigenicity of breast cancer stem cells.Stem cell research & therapy · 2024Article
- Nanofiber-microwell cell culture system for spatially patterned differentiation of pluripotent stem cells in 3D.Materials today. Bio · 2024Article
- Deep Learning Powered Identification of Differentiated Early Mesoderm Cells from Pluripotent Stem Cells.Cells · 2024Article
- Mass production of lumenogenic human embryoid bodies and functional cardiospheres using in-air-generated microcapsules.Nature communications · 2023Article
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- YAP at the Crossroads of Biomechanics and Drug Resistance in Human Cancer.International journal of molecular sciences · 2023Review
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- Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons.bioRxiv : the preprint server for biology · 2023Article
- Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm.Protein & cell · 2023Article
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- Opportunities and challenges in stem cell therapy in cardiovascular diseases: Position standing in 2022.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2022Review
- Forces in stem cells and cancer stem cells.Cells & development · 2022Review
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 3 institutions in 2 countries.
Funding
Abstract
Mammalian inner cell mass cells undergo lineage-specific differentiation into germ layers of endoderm, mesoderm and ectoderm during gastrulation. It has been a long-standing challenge in developmental biology to replicate these organized germ layer patterns in culture. Here we present a method of generating organized germ layers from a single mouse embryonic stem cell cultured in a soft fibrin matrix. Spatial organization of germ layers is regulated by cortical tension of the colony, matrix dimensionality and softness, and cell-cell adhesion. Remarkably, anchorage of the embryoid colony from the 3D matrix to collagen-1-coated 2D substrates of ~1 kPa results in self-organization of all three germ layers: ectoderm on the outside layer, mesoderm in the middle and endoderm at the centre of the colony, reminiscent of generalized gastrulating chordate embryos. These results suggest that mechanical forces via cell-matrix and cell-cell interactions are crucial in spatial organization of germ layers during mammalian gastrulation. This new in vitro method could be used to gain insights on the mechanisms responsible for the regulation of germ layer formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.