ArticleMolecular & cellular proteomics : MCP2014
Profiling the secretome and extracellular proteome of the potato late blight pathogen Phytophthora infestans.
Article in Molecular & cellular proteomics : MCP, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed, 81 citations in OpenAlex.
- Isolation and proteomic analysis of intracellular vesicles from the potato late blight pathogen Phytophthora infestans.Scientific reports · 2026Article
- Pathogen-inspired engineering of plant protease enhances late blight resistance.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Oomycetes manipulate plant innate immunity through galacturonide oxidases.Nature communications · 2025Article
- Profiling the Genomes and Secreted Effector Proteins inJournal of fungi (Basel, Switzerland) · 2025Article
- Application of Proteomic Methods in Oomycete Biology.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Carbon and Nitrogen Sources Influence Parasitic Responsiveness inJournal of fungi (Basel, Switzerland) · 2024Article
- The function of plant PR1 and other members of the CAP protein superfamily in plant-pathogen interactions.Molecular plant pathology · 2023Review
- Omics Approaches in Invasion Biology: Understanding Mechanisms and Impacts on Ecological Health.Plants (Basel, Switzerland) · 2023Review
- Genome analysis ofFrontiers in microbiology · 2023Article
- The root pathogenFrontiers in plant science · 2023Article
- An in-planta comparative study of Plasmopara viticola proteome reveals different infection strategies towards susceptible and Rpv3-mediated resistance hosts.Scientific reports · 2022Article
- Secretome Analysis ofFrontiers in microbiology · 2022Article
- Chromosome-level assembly of theFrontiers in microbiology · 2022Article
- "Core" RxLR effectors in phytopathogenic oomycetes: A promising way to breeding for durable resistance in plants?Virulence · 2021Article
- Mining oomycete proteomes for metalloproteases leads to identification of candidate virulence factors in Phytophthora infestans.Molecular plant pathology · 2021Article
- Genome of the destructive oomycete Phytophthora cinnamomi provides insights into its pathogenicity and adaptive potential.BMC genomics · 2021Article
- Defense and Offense Strategies: The Role of Aspartic Proteases in Plant-Pathogen Interactions.Biology · 2021Review
- Pathogen-Mediated Stomatal Opening: A Previously Overlooked Pathogenicity Strategy in the Oomycete PathogenFrontiers in plant science · 2021Article
- Gene Validation and Remodelling Using Proteogenomics ofFrontiers in microbiology · 2021Article
- Developmental Modulation of Root Cell Wall Architecture Confers Resistance to an Oomycete Pathogen.Current biology : CB · 2020Article
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oomycetes are filamentous organisms that cause notorious diseases, several of which have a high economic impact. Well known is Phytophthora infestans, the causal agent of potato late blight. Previously, in silico analyses of the genome and transcriptome of P. infestans resulted in the annotation of a large number of genes encoding proteins with an N-terminal signal peptide. This set is collectively referred to as the secretome and comprises proteins involved in, for example, cell wall growth and modification, proteolytic processes, and the promotion of successful invasion of plant cells. So far, proteomic profiling in oomycetes was primarily focused on subcellular, intracellular or cell wall fractions; the extracellular proteome has not been studied systematically. Here we present the first comprehensive characterization of the in vivo secretome and extracellular proteome of P. infestans. We have used mass spectrometry to analyze P. infestans proteins present in seven different growth media with mycelial cultures and this resulted in the consistent identification of over two hundred proteins. Gene ontology classification pinpointed proteins involved in cell wall modifications, pathogenesis, defense responses, and proteolytic processes. Moreover, we found members of the RXLR and CRN effector families as well as several proteins lacking an obvious signal peptide. The latter were confirmed to be bona fide extracellular proteins and this suggests that, similar to other organisms, oomycetes exploit non-conventional secretion mechanisms to transfer certain proteins to the extracellular environment.
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