ArticleMolecular & cellular proteomics : MCP2014
Deciphering thylakoid sub-compartments using a mass spectrometry-based approach.
Article in Molecular & cellular proteomics : MCP, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers, 1 of them a synthesis that pooled it.
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51 citing papers in PubMed, 1 synthesis or guideline pooled it, 101 citations in OpenAlex.
- Assessing sub-cellular resolution in spatial proteomics experiments.Current opinion in chemical biology · 2019Pooled it
- From proton microdomains to protein migration: hypothesis for ΔpH-controlled PSII relocation.Photosynthesis research · 2026Article
- Lipid metabolism and contact site homologs are present at the chloroplast envelope-thylakoid interface.Nature communications · 2026Article
- APE1 is involved in the stabilization/formation of the RC47 complex of PSII in oxygenic prototrophic organisms.Plant physiology · 2026Article
- Thylakostasis: key factors in thylakoid membrane organization with emphasis on biogenesis and remodeling proteins in vascular plants.Plant & cell physiology · 2025Review
- Evaluating the roles of drift and selection in trait loss along an elevational gradient.Evolution; international journal of organic evolution · 2025Article
- Identification of STN7-associated leaf senescence networks in thylakoid membranes of Arabidopsis.iScience · 2025Article
- Defining the heterogeneous composition of Arabidopsis thylakoid membrane.The Plant journal : for cell and molecular biology · 2025Article
- Localization of proteins involved in the biogenesis and repair of the photosynthetic apparatus to thylakoid subdomains inPlant direct · 2024Article
- A pgr5 suppressor screen uncovers two distinct suppression mechanisms and links cytochrome b6f complex stability to PGR5.The Plant cell · 2024Article
- CO-EXPRESSED WITH PSI ASSEMBLY1 (CEPA1) is a photosystem I assembly factor in Arabidopsis.The Plant cell · 2024Article
- The Main Functions of Plastids.Methods in molecular biology (Clifton, N.J.) · 2024Review
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- Bicarbonate activation of the monomeric photosystem II-PsbS/Psb27 complex.Plant physiology · 2023Article
- Loss of CpFTSY Reduces Photosynthetic Performance and Affects Insertion of PsaC of PSI in Diatoms.Plant & cell physiology · 2023Article
- CGL160-mediated recruitment of the coupling factor CF1 is required for efficient thylakoid ATP synthase assembly, photosynthesis, and chloroplast development in Arabidopsis.The Plant cell · 2023Article
- TOR kinase, a GPS in the complex nutrient and hormonal signaling networks to guide plant growth and development.Journal of experimental botany · 2022Review
- Friend or foe: Hybrid proline-rich proteins determine how plants respond to beneficial and pathogenic microbes.Plant physiology · 2022Article
- Dissecting the Chloroplast Proteome of the Potato (Plants (Basel, Switzerland) · 2022Article
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Authors and funding
14 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photosynthesis has shaped atmospheric and ocean chemistries and probably changed the climate as well, as oxygen is released from water as part of the photosynthetic process. In photosynthetic eukaryotes, this process occurs in the chloroplast, an organelle containing the most abundant biological membrane, the thylakoids. The thylakoids of plants and some green algae are structurally inhomogeneous, consisting of two main domains: the grana, which are piles of membranes gathered by stacking forces, and the stroma-lamellae, which are unstacked thylakoids connecting the grana. The major photosynthetic complexes are unevenly distributed within these compartments because of steric and electrostatic constraints. Although proteomic analysis of thylakoids has been instrumental to define its protein components, no extensive proteomic study of subthylakoid localization of proteins in the BBY (grana) and the stroma-lamellae fractions has been achieved so far. To fill this gap, we performed a complete survey of the protein composition of these thylakoid subcompartments using thylakoid membrane fractionations. We employed semiquantitative proteomics coupled with a data analysis pipeline and manual annotation to differentiate genuine BBY and stroma-lamellae proteins from possible contaminants. About 300 thylakoid (or potentially thylakoid) proteins were shown to be enriched in either the BBY or the stroma-lamellae fractions. Overall, present findings corroborate previous observations obtained for photosynthetic proteins that used nonproteomic approaches. The originality of the present proteomic relies in the identification of photosynthetic proteins whose differential distribution in the thylakoid subcompartments might explain already observed phenomenon such as LHCII docking. Besides, from the present localization results we can suggest new molecular actors for photosynthesis-linked activities. For instance, most PsbP-like subunits being differently localized in stroma-lamellae, these proteins could be linked to the PSI-NDH complex in the context of cyclic electron flow around PSI. In addition, we could identify about a hundred new likely minor thylakoid (or chloroplast) proteins, some of them being potential regulators of the chloroplast physiology.
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