ArticleJournal of molecular cell biology2014
The conserved ubiquitin-like protein Hub1 plays a critical role in splicing in human cells.
Article in Journal of molecular cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Mechanism of alternative splicing of yeast HEH1 through competing 5' splice sites.Nature communications · 2025Article
- Integrative analysis of Hub1 overexpression: driving transcriptional reprogramming and alternative splicing in Saccharomyces cerevisiae.BMC genomics · 2025Article
- UBL5 and Its Role in Viral Infections.Viruses · 2024Review
- Structural insights into human exon-defined spliceosome prior to activation.Cell research · 2024Article
- The Ubiquitin-like Proteins ofBiomolecules · 2023Review
- Regulation of Pre-mRNA Splicing: Indispensable Role of Post-Translational Modifications of Splicing Factors.Life (Basel, Switzerland) · 2023Review
- The ubiquitin-like protein Hub1/UBL-5 functions in pre-mRNA splicing in Caenorhabditis elegans.FEBS letters · 2023Article
- Computer- and NMR-Aided Design of Small-Molecule Inhibitors of the Hub1 Protein.Molecules (Basel, Switzerland) · 2022Article
- Splicing of branchpoint-distant exons is promoted by Cactin, Tls1 and the ubiquitin-fold-activated Sde2.Nucleic acids research · 2022Article
- Broader roles of the ubiquitin-like protein Hub1 indicated by its yeast two-hybrid interactors.microPublication biology · 2022Article
- Ubiquitination and Ubiquitin-Like Modifications as Mediators of Alternative Pre-mRNA Splicing inFrontiers in plant science · 2022Review
- UBL5/Hub1: An Atypical Ubiquitin-Like Protein with a Typical Role as a Stress-Responsive Regulator.International journal of molecular sciences · 2021Review
- Ubiquitin-Like protein 5 interacts with the silencing suppressor p3 of rice stripe virus and mediates its degradation through the 26S proteasome pathway.PLoS pathogens · 2020Article
- Identification of Sugarcane Host Factors Interacting with the 6K2 Protein of theInternational journal of molecular sciences · 2019Article
- AtUBL5 regulates growth and development through pre-mRNA splicing in Arabidopsis thaliana.PloS one · 2019Article
- Structures of the human pre-catalytic spliceosome and its precursor spliceosome.Cell research · 2018Article
- Intron specificity in pre-mRNA splicing.Current genetics · 2018Review
- Article
- Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing.The EMBO journal · 2018Article
- Human MFAP1 is a cryptic ortholog of the Saccharomyces cerevisiae Spp381 splicing factor.BMC evolutionary biology · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Different from canonical ubiquitin-like proteins, Hub1 does not form covalent conjugates with substrates but binds proteins non-covalently. In Saccharomyces cerevisiae, Hub1 associates with spliceosomes and mediates alternative splicing of SRC1, without affecting pre-mRNA splicing generally. Human Hub1 is highly similar to its yeast homolog, but its cellular function remains largely unexplored. Here, we show that human Hub1 binds to the spliceosomal protein Snu66 as in yeast; however, unlike its S. cerevisiae homolog, human Hub1 is essential for viability. Prolonged in vivo depletion of human Hub1 leads to various cellular defects, including splicing speckle abnormalities, partial nuclear retention of mRNAs, mitotic catastrophe, and consequently cell death by apoptosis. Early consequences of Hub1 depletion are severe splicing defects, however, only for specific splice sites leading to exon skipping and intron retention. Thus, the ubiquitin-like protein Hub1 is not a canonical spliceosomal factor needed generally for splicing, but rather a modulator of spliceosome performance and facilitator of alternative splicing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.