ArticleProceedings of the National Academy of Sciences of the United States of America2014
Use of transmission electron microscopy to identify nanocrystals of challenging protein targets.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Towards time-resolved MicroED grid preparation using mix-and-inject gas dynamic virtual nozzles.IUCrJ · 2026Article
- Comprehensive microcrystal electron diffraction sample preparation for cryo-EM.Nature protocols · 2025Review
- Review
- Non-invasive nanoscale imaging of protein micro- and nanocrystals for screening crystallization conditions.Journal of applied crystallography · 2024Article
- Structural basis of transcription: RNA polymerase II substrate binding and metal coordination using a free-electron laser.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Reaching the potential of electron diffraction.Cell reports. Physical science · 2024Article
- Formulation Approaches to Crystalline Status Modification for Carotenoids: Impacts on Dissolution, Stability, Bioavailability, and Bioactivities.Pharmaceutics · 2023Review
- Sample Preparation for Time-Resolved Serial Crystallography: Practical Considerations.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Determination of Conformational and Functional Stability of Potential Plague Vaccine Candidate in Formulation.Vaccines · 2022Article
- Review
- An Overview of Microcrystal Electron Diffraction (MicroED).Annual review of biochemistry · 2021Review
- MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography.Nature communications · 2021Article
- Macromolecular crystallography using microcrystal electron diffraction.Acta crystallographica. Section D, Structural biology · 2021Review
- MicroED Sample Preparation and Data Collection For Protein Crystals.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Detection of Microcrystals for CryoEM.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Rapid screening ofJournal of applied crystallography · 2020Article
- The complementarity of serial femtosecond crystallography and MicroED for structure determination from microcrystals.Current opinion in structural biology · 2019Review
- The cryo-EM method microcrystal electron diffraction (MicroED).Nature methods · 2019Review
- Transcription with a laser: Radiation-damage-free diffraction of RNA Polymerase II crystals.Methods (San Diego, Calif.) · 2019Article
- Nanobeam precession-assisted 3D electron diffraction reveals a new polymorph of hen egg-white lysozyme.IUCrJ · 2019Article
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16 authors.
Funding
Abstract
The current practice for identifying crystal hits for X-ray crystallography relies on optical microscopy techniques that are limited to detecting crystals no smaller than 5 μm. Because of these limitations, nanometer-sized protein crystals cannot be distinguished from common amorphous precipitates, and therefore go unnoticed during screening. These crystals would be ideal candidates for further optimization or for femtosecond X-ray protein nanocrystallography. The latter technique offers the possibility to solve high-resolution structures using submicron crystals. Transmission electron microscopy (TEM) was used to visualize nanocrystals (NCs) found in crystallization drops that would classically not be considered as "hits." We found that protein NCs were readily detected in all samples tested, including multiprotein complexes and membrane proteins. NC quality was evaluated by TEM visualization of lattices, and diffraction quality was validated by experiments in an X-ray free electron laser.
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