ArticleAmerican journal of physiology. Renal physiology2014
Effects of biomechanical forces on signaling in the cortical collecting duct (CCD).
Article in American journal of physiology. Renal physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Engineering scalable vascularized kidney organoids for in vivo glomerular filtration with human endothelial integration.npj biomedical innovations · 2026Article
- Going with the flow: New insights regarding flow induced KPhysiological reports · 2024Review
- Expression and localization of the mechanosensitive/osmosensitive ion channel TMEM63B in the mouse urinary tract.Physiological reports · 2024Article
- Prostanoid receptors in hyperfiltration-mediated glomerular injury: Novel agonists and antagonists reveal opposing roles for EP2 and EP4 receptors.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Kidney-on-a-Chip: Mechanical Stimulation and Sensor Integration.Sensors (Basel, Switzerland) · 2022Review
- Glomerular Biomechanical Stress and Lipid Mediators during Cellular Changes Leading to Chronic Kidney Disease.Biomedicines · 2022Review
- Unilateral Nephrectomy Stimulates ERK and Is Associated With Enhanced Na Transport.Frontiers in physiology · 2021Article
- Role of Shear Stress on Renal Proximal Tubular Cells for Nephrotoxicity Assays.Journal of toxicology · 2021Review
- Effect of luminal flow on doming of mpkCCD cells in a 3D perfusable kidney cortical collecting duct model.American journal of physiology. Cell physiology · 2020Article
- Sensing of tubular flow and renal electrolyte transport.Nature reviews. Nephrology · 2020Review
- Pulsatile flow through idealized renal tubules: Fluid-structure interaction and dynamic pathologies.Mathematical biosciences and engineering : MBE · 2019Article
- Expression and distribution of PIEZO1 in the mouse urinary tract.American journal of physiology. Renal physiology · 2019Article
- Effect of fluid shear stress onBiomicrofluidics · 2018Article
- Mechanotransduction signaling in podocytes from fluid flow shear stress.American journal of physiology. Renal physiology · 2018Article
- Hyperfiltration-associated biomechanical forces in glomerular injury and response: Potential role for eicosanoids.Prostaglandins & other lipid mediators · 2017Review
- Intravital multiphoton microscopy as a tool for studying renal physiology and pathophysiology.Methods (San Diego, Calif.) · 2017Review
- Surgical preparation of rats and mice for intravital microscopic imaging of abdominal organs.Methods (San Diego, Calif.) · 2017Article
- The mechanosensitive BKα/β1 channel localizes to cilia of principal cells in rabbit cortical collecting duct (CCD).American journal of physiology. Renal physiology · 2017Article
- An unexpected journey: conceptual evolution of mechanoregulated potassium transport in the distal nephron.American journal of physiology. Cell physiology · 2016Review
- Cholesterol affects flow-stimulated cyclooxygenase-2 expression and prostanoid secretion in the cortical collecting duct.American journal of physiology. Renal physiology · 2015Article
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
Abstract
An increase in tubular fluid flow rate (TFF) stimulates Na reabsorption and K secretion in the cortical collecting duct (CCD) and subjects cells therein to biomechanical forces including fluid shear stress (FSS) and circumferential stretch (CS). Intracellular MAPK and extracellular autocrine/paracrine PGE2 signaling regulate cation transport in the CCD and, at least in other systems, are affected by biomechanical forces. We hypothesized that FSS and CS differentially affect MAPK signaling and PGE2 release to modulate cation transport in the CCD. To validate that CS is a physiological force in vivo, we applied the intravital microscopic approach to rodent kidneys in vivo to show that saline or furosemide injection led to a 46.5 ± 2.0 or 170 ± 32% increase, respectively, in distal tubular diameter. Next, murine CCD (mpkCCD) cells were grown on glass or silicone coated with collagen type IV and subjected to 0 or 0.4 dyne/cm(2) of FSS or 10% CS, respectively, forces chosen based on prior biomechanical modeling of ex vivo microperfused CCDs. Cells exposed to FSS expressed an approximately twofold greater abundance of phospho(p)-ERK and p-p38 vs. static cells, while CS did not alter p-p38 and p-ERK expression compared with unstretched controls. FSS induced whereas CS reduced PGE2 release by ∼40%. In conclusion, FSS and CS differentially affect ERK and p38 activation and PGE2 release in a cell culture model of the CD. We speculate that TFF differentially regulates biomechanical signaling and, in turn, cation transport in the CCD.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.