ArticleJournal of clinical microbiology2014
A rapid and high-throughput screening approach for methicillin-resistant Staphylococcus aureus based on the combination of two different real-time PCR assays.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Panton-Valentine Leukocidin-Encoding Methicillin-Resistant Staphylococcus aureus, the Netherlands, 2023-2024Emerging infectious diseases · 2026Article
- Evaluation of the Performance of Novel Gram-Negative and Gram-Positive Sepsis Panels for the Rapid Diagnosis of Bloodstream Infections.Diagnostics (Basel, Switzerland) · 2026Article
- Antimicrobial hydrogel foam dressing with controlled release of gallium maltolate for infection control in chronic wounds.Bioactive materials · 2024Article
- Experimental colonization with H. hepaticus, S. aureus and R. pneumotropicus does not influence the metabolic response to high-fat diet or incretin-analogues in wildtype SOPF mice.Molecular metabolism · 2024Article
- Recent Advances in Nanozyme-Mediated Strategies for Pathogen Detection and Control.International journal of molecular sciences · 2023Review
- A pseudo-outbreak of MRSA due to laboratory contamination related to MRSA carriage of a laboratory staff member.Antimicrobial resistance and infection control · 2023Article
- Development of a Real-Time Recombinase-Aided Amplification Method to Rapidly Detect Methicillin-ResistantMicroorganisms · 2022Article
- Methicillin-resistant Staphylococcus argenteus in the Netherlands: not a new arrival.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- Acquisition of multidrug-resistant bacteria and encoding genes among French pilgrims during the 2017 and 2018 Hajj.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- HumanMicroorganisms · 2020Review
- Effect of gallium maltolate on a model of chronic, infected equine distal limb wounds.PloS one · 2020Article
- Active surveillance for highly resistant microorganisms in patients with prolonged hospitalization.Antimicrobial resistance and infection control · 2020Observational
- Validating the AMRFinder Tool and Resistance Gene Database by Using Antimicrobial Resistance Genotype-Phenotype Correlations in a Collection of Isolates.Antimicrobial agents and chemotherapy · 2019Article
- Nanomaterial-based optical and electrochemical techniques for detection of methicillin-resistant Staphylococcus aureus: a review.Mikrochimica acta · 2019Review
- Development and Validation of a Reference Data Set for AssigningFrontiers in cellular and infection microbiology · 2019Article
- High frequency of occupied attB regions in Norwegian Staphylococcus aureus isolates supports a two-step MRSA screening algorithm.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Article
- Beyond Blood Culture and Gram Stain Analysis: A Review of Molecular Techniques for the Early Detection of Bacteremia in Surgical Patients.Surgical infections · 2016Review
- Detection of mecA- and mecC-Positive Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates by the New Xpert MRSA Gen 3 PCR Assay.Journal of clinical microbiology · 2016Article
- A Novel Staphylococcal Cassette Chromosome mec Type XI Primer for Detection of mecC-Harboring Methicillin-Resistant Staphylococcus aureus Directly from Screening Specimens.Journal of clinical microbiology · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen that has been responsible for major nosocomial epidemics worldwide. For infection control programs, rapid and adequate detection of MRSA is of great importance. We developed a rapid and high-throughput molecular screening approach that consists of an overnight selective broth enrichment, followed by mecA, mecC, and S. aureus-specific (SA442 gene) real-time PCR assays, with subsequent confirmation using a staphylococcal cassette chromosome mec element (SCCmec)-orfX-based real-time PCR assay (GeneOhm MRSA assay) and culture. Here, the results of the screening approach over a 2-year period are presented. During this period, a total of 13,387 samples were analyzed for the presence of MRSA, 2.6% of which were reported as MRSA positive. No MRSA isolates carrying the mecC gene were detected during this study. Based on the results of the real-time PCR assays only, 95.2% of the samples could be reported as negative within 24 h. Furthermore, the performance of these real-time PCR assays was evaluated using a set of 104 assorted MRSA isolates, which demonstrated high sensitivity for both the combination of mecA and mecC with SA442 and the BD GeneOhm MRSA assay (98.1% and 97.1%, respectively). This molecular screening approach proved to be an accurate method for obtaining reliable negative results within 24 h after arrival at the laboratory and contributes to improvement of infection control programs, especially in areas with a low MRSA prevalence.
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