ArticleJournal of clinical microbiology2014
Clinical utility of droplet digital PCR for human cytomegalovirus.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
49 citing papers in PubMed.
- From Surfaces to Spillover: Environmental Persistence and Indirect Transmission of Influenza A(H3N8) Virus.Microorganisms · 2025Article
- Varicella-Zoster Virus-Specific Cell-Mediated Immune Response Kinetics and Latent Viral Load Depending on Aging.Journal of medical virology · 2025Article
- Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis.Diagnostics (Basel, Switzerland) · 2024Review
- Human trophoblast stem cells restrict human cytomegalovirus replication.Journal of virology · 2024Article
- Decoding human cytomegalovirus for the development of innovative diagnostics to detect congenital infection.Pediatric research · 2024Review
- Droplet Digital PCR (ddPCR) Does Not Enhance the Sensitivity of Detection of Cytomegalovirus (CMV) DNA in Newborn Dried Blood Spots Evaluated in the Context of Newborn Congenital CMV (cCMV) Screening.International journal of neonatal screening · 2023Article
- Digital PCR Discriminates between SARS-CoV-2 Omicron Variants and Immune Escape Mutations.Microbiology spectrum · 2023Article
- Heteroresistance ofBiology · 2023Article
- Detection ofPathogens (Basel, Switzerland) · 2023Article
- Evaluation of the Association Between Congenital Cytomegalovirus Infection and Pediatric Acute Lymphoblastic Leukemia.JAMA network open · 2023Article
- Droplet digital PCR application for the detection of SARS-CoV-2 in air sample.Frontiers in public health · 2023Article
- Evaluation of droplet digital qRT-PCR (dd qRT-PCR) for quantification of SARS CoV-2 RNA in stool and urine specimens of COVID-19 patients.Frontiers in medicine · 2023Article
- Cytomegalovirus in children undergoing haematopoietic stem cell transplantation: a diagnostic and therapeutic approach to antiviral resistance.Frontiers in pediatrics · 2023Review
- Ready for new waves: optimizing SARS-CoV-2 variants monitoring in pooled samples with droplet digital PCR.Frontiers in public health · 2023Article
- Pathogen load and species monitored by droplet digital PCR in patients with bloodstream infections: A prospective case series study.BMC infectious diseases · 2022Article
- Sponge Whirl-Pak Sampling Method and Droplet Digital RT-PCR Assay for Monitoring of SARS-CoV-2 on Surfaces in Public and Working Environments.International journal of environmental research and public health · 2022Article
- Ultrasensitive detection and quantification of viral nucleic acids with Raindance droplet digital PCR (ddPCR).Methods (San Diego, Calif.) · 2022Article
- Laboratory diagnostic testing for cytomegalovirus infection in solid organ transplant patients.Korean journal of transplantation · 2022Review
- The Viral Load of Human Cytomegalovirus Infection in Children following Hematopoietic Stem Cell Transplant by Chip Digital PCR.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2022Article
- Droplet digital PCR as a tool for investigating dynamics of cryptic symbionts.Ecology and evolution · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human cytomegalovirus (CMV) has historically been the major infectious cause of morbidity and mortality among patients receiving hematopoietic cell or organ transplant. Standard care in a transplant setting involves frequent monitoring of CMV viral load over weeks to months to determine when antiviral treatment may be required. Quantitative PCR (qPCR) is the standard molecular diagnostic method for monitoring. Recently, digital PCR (dPCR) has shown promise in viral diagnostics, although current dPCR systems have lower throughput than qPCR systems. Here, we compare qPCR and droplet digital PCR (ddPCR) for CMV detection in patient plasma samples. Droplet digital PCR exhibits increased precision over qPCR at viral loads of ≥4 log10 with equivalent sensitivity. However, retrospective analysis of longitudinal samples from transplant patients with CMV viral loads near therapeutic thresholds did not provide evidence that the improved precision of ddPCR would be of clinical benefit. Given the throughput advantages of current qPCR systems, a widespread switch to dPCR for CMV monitoring would appear premature.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.