ArticleProtein & cell2014
Development of an activity-directed selection system enabled significant improvement of the carboxylation efficiency of Rubisco.
Article in Protein & cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 79 citations in OpenAlex.
- The fitness landscape of a form II rubisco in a photosynthetic bacterium guides engineering of oxygen tolerance.Science advances · 2026Article
- High-Throughput Detection of Cyanobacterial Form I Rubisco Assembly.ACS synthetic biology · 2026Article
- Replacement of large subunit N terminus enabled biogenesis of different plant Rubiscos in E. coli.Plant biotechnology journal · 2025Article
- In vivo directed evolution of an ultrafast Rubisco from a semianaerobic environment imparts oxygen resistance.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Discussion of the possibility of increasing the efficiency of photosynthesis using genetic and metabolic engineering methods.Biodesign research · 2025Review
- Molecular mechanisms regulating glucose metabolism in quinoa (Chenopodium quinoa Willd.) seeds under drought stress.BMC plant biology · 2024Article
- Engineering Rubisco to enhance COSynthetic and systems biotechnology · 2024Review
- Integrative Physiological, Transcriptome, and Proteome Analyses Provide Insights into the Photosynthetic Changes in Maize in a Maize-Peanut Intercropping System.Plants (Basel, Switzerland) · 2023Article
- Natural carbon fixation and advances in synthetic engineering for redesigning and creating new fixation pathways.Journal of advanced research · 2023Review
- Cell-free expression of RuBisCO for ATP production in the synthetic cells.Synthetic biology (Oxford, England) · 2023Article
- Discovery of a readily heterologously expressed Rubisco from the deep sea with potential for COBioresources and bioprocessing · 2021Article
- Impairing photorespiration increases photosynthetic conversion of COBioresources and bioprocessing · 2021Article
- Engineering the Calvin-Benson-Bassham cycle and hydrogen utilization pathway of Ralstonia eutropha for improved autotrophic growth and polyhydroxybutyrate production.Microbial cell factories · 2020Article
- Functional reconstitution of a bacterial COeLife · 2020Article
- Recent Advances in Developing Artificial Autotrophic Microorganism for Reinforcing COFrontiers in microbiology · 2020Review
- Engineering Photosynthetic Bioprocesses for Sustainable Chemical Production: A Review.Frontiers in bioengineering and biotechnology · 2020Review
- Directed Evolution of an Improved Rubisco; In Vitro Analyses to Decipher Fact from Fiction.International journal of molecular sciences · 2019Article
- Exploring natural variation of photosynthesis in a site-specific manner: evolution, progress, and prospects.Planta · 2019Review
- Development of an autotrophic fermentation technique for the production of fatty acids using an engineered Ralstonia eutropha cell factory.Journal of industrial microbiology & biotechnology · 2019Article
- Applications of Protein Engineering and Directed Evolution in Plant Research.Plant physiology · 2019Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photosynthetic CO(2) fixation is the ultimate source of organic carbon on earth and thus is essential for crop production and carbon sequestration. Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalyzes the first step of photosynthetic CO(2) fixation. However, the extreme low carboxylation efficiency of Rubisco makes it the most attractive target for improving photosynthetic efficiency. Extensive studies have focused on re-engineering a more efficient enzyme, but the effort has been impeded by the limited understanding of its structure-function relationships and the lack of an efficient selection system towards its activity. To address the unsuccessful molecular engineering of Rubisco, we developed an Escherichia coli-based activity-directed selection system which links the growth of host cell solely to the Rubisco activity therein. A Synechococcus sp. PCC7002 Rubisco mutant with E49V and D82G substitutions in the small subunit was selected from a total of 15,000 mutants by one round of evolution. This mutant showed an 85% increase in specific carboxylation activity and a 45% improvement in catalytic efficiency towards CO(2). The small-subunit E49V mutation was speculated to influence holoenzyme catalysis through interaction with the large-subunit Q225. This interaction is conserved among various Rubisco from higher plants and Chlamydomonas reinhardtii. Knowledge of these might provide clues for engineering Rubisco from higher plants, with the potential of increasing the crop yield.
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