ArticleBlood2014
Dissection of vertebrate hematopoiesis using zebrafish thrombopoietin.
Article in Blood, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- A new role for erythropoietin in the homeostasis of red blood cells.Communications biology · 2024Article
- Establishment of a Bernard-Soulier syndrome model in zebrafish.Haematologica · 2022Article
- Haematopoiesis in Zebrafish (Danio Rerio).Frontiers in immunology · 2022Review
- Phosphatidylinositol-3 kinase signaling controls survival and stemness of hematopoietic stem and progenitor cells.Oncogene · 2021Article
- son is necessary for proper vertebrate blood development.PloS one · 2021Article
- Differential Requirement ofFrontiers in cell and developmental biology · 2021Article
- Zebrafish Kit ligands cooperate with erythropoietin to promote erythroid cell expansion.Blood advances · 2020Article
- Evidence for the loss of plasminogen receptor KT gene in chicken.Immunogenetics · 2020Article
- Zebrafish for thrombocytopoiesis- and hemostasis-related researches and disorders.Blood science (Baltimore, Md.) · 2020Review
- Characterisation of Thrombocytes in Osteichthyes.Journal of veterinary research · 2019Article
- Nfe2 is dispensable for early but required for adult thrombocyte formation and function in zebrafish.Blood advances · 2018Article
- Hematopoietic Cytokine Gene Duplication in Zebrafish Erythroid and Myeloid Lineages.Frontiers in cell and developmental biology · 2018Review
- Article
- Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells (HSPCs) in Developing Zebrafish Embryos.Journal of visualized experiments : JoVE · 2017Article
- Thrombopoietic stimulating activity of rhTyrRS (Y341A).Scientific reports · 2017Article
- Establishment of a congenital amegakaryocytic thrombocytopenia model and a thrombocyte-specific reporter line in zebrafish.Leukemia · 2017Article
- Zebrafish Caudal Haematopoietic Embryonic Stromal Tissue (CHEST) Cells Support Haematopoiesis.Scientific reports · 2017Article
- The Function of Fish Cytokines.Biology · 2016Review
- Ex vivo tools for the clonal analysis of zebrafish hematopoiesis.Nature protocols · 2016Article
- Thrombopoietin induces production of nucleated thrombocytes from liver cells in Xenopus laevis.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
In nonmammalian vertebrates, the functional units of hemostasis are thrombocytes. Thrombocytes are thought to arise from bipotent thrombocytic/erythroid progenitors (TEPs). TEPs have been experimentally demonstrated in avian models of hematopoiesis, and mammals possess functional equivalents known as megakaryocyte/erythroid progenitors (MEPs). However, the presence of TEPs in teleosts has only been speculated. To identify and prospectively isolate TEPs, we identified, cloned, and generated recombinant zebrafish thrombopoietin (Tpo). Tpo mRNA expanded itga2b:GFP(+) (cd41:GFP(+)) thrombocytes as well as hematopoietic stem and progenitor cells (HSPCs) in the zebrafish embryo. Utilizing Tpo in clonal methylcellulose assays, we describe for the first time the prospective isolation and characterization of TEPs from transgenic zebrafish. Combinatorial use of zebrafish Tpo, erythropoietin, and granulocyte colony stimulating factor (Gcsf) allowed the investigation of HSPCs responsible for erythro-, myelo-, and thrombo-poietic differentiation. Utilizing these assays allowed the visualization and differentiation of hematopoietic progenitors ex vivo in real-time with time-lapse and high-throughput microscopy, allowing analyses of their clonogenic and proliferative capacity. These studies indicate that the functional role of Tpo in the differentiation of thrombocytes from HSPCs is well conserved among vertebrate organisms, positing the zebrafish as an excellent model to investigate diseases caused by dysregulated erythro- and thrombo-poietic differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.