ArticleNature communications2014
In vivo imaging of specific drug-target binding at subcellular resolution.
Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed.
- Pinhole engineering based enhanced resolution (PEER) for fluorescence lifetime imaging microscopy.Communications biology · 2026Article
- Pinhole Engineering based Enhanced Resolution (PEER) for Fluorescence Lifetime Imaging Microscopy.Research square · 2025Article
- Imaging the Binding Between Dasatinib and Its Target Protein in Living Cells Using an SLP Tag System on Intracellular Compartments.International journal of molecular sciences · 2025Article
- PARP trapping is governed by the PARP inhibitor dissociation rate constant.Cell chemical biology · 2024Article
- Advanced imaging techniques for tracking drug dynamics at the subcellular level.Advanced drug delivery reviews · 2023Review
- Polyethylene Glycol 3350 (PEG 3350) as a Practical Vehicle for Rapid Reconstitution of PARPi-FL Formulations for Clinical Use.Molecular imaging and biology · 2023Article
- Monitoring drug-target interactions through target engagement-mediated amplification on arrays and in situ.Nucleic acids research · 2022Article
- Synthesis of fluorescently labeled pyrazole derivative induceing a triple response inJournal of pesticide science · 2022Article
- DTI-BERT: Identifying Drug-Target Interactions in Cellular Networking Based on BERT and Deep Learning Method.Frontiers in genetics · 2022Article
- Bright and stable luminescent probes for target engagement profiling in live cells.Nature chemical biology · 2021Article
- PARP1: A Potential Molecular Marker to Identify Cancer During Colposcopy Procedures.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2021Article
- Quantification of Cellular Drug Biodistribution Addresses Challenges in EvaluatingAdvanced therapeutics · 2021Article
- Recent advances in nonlinear microscopy: Deep insights and polarized revelations.The international journal of biochemistry & cell biology · 2021Review
- Understanding the in vivo Fate of Advanced Materials by Imaging.Advanced functional materials · 2020Article
- Quantifying drug tissue biodistribution by integrating high content screening with deep-learning analysis.Scientific reports · 2020Article
- Extended dynamic range imaging for noise mitigation in fluorescence anisotropy imaging.Journal of biomedical optics · 2020Article
- Fluorescence-guided resection of tumors in mouse models of oral cancer.Scientific reports · 2020Article
- Importance of Quantifying Drug-Target Engagement in Cells.ACS medicinal chemistry letters · 2020Article
- Frontiers in Intravital Multiphoton Microscopy of Cancer.Cancer reports (Hoboken, N.J.) · 2020Review
- Poly(ADP-Ribose)Polymerase (PARP) Inhibitors and Radiation Therapy.Frontiers in pharmacology · 2020Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The possibility of measuring binding of small-molecule drugs to desired targets in live cells could provide a better understanding of drug action. However, current approaches mostly yield static data, require lysis or rely on indirect assays and thus often provide an incomplete understanding of drug action. Here, we present a multiphoton fluorescence anisotropy microscopy live cell imaging technique to measure and map drug-target interaction in real time at subcellular resolution. This approach is generally applicable using any fluorescently labelled drug and enables high-resolution spatial and temporal mapping of bound and unbound drug distribution. To illustrate our approach we measure intracellular target engagement of the chemotherapeutic Olaparib, a poly(ADP-ribose) polymerase inhibitor, in live cells and within a tumour in vivo. These results are the first generalizable approach to directly measure drug-target binding in vivo and present a promising tool to enhance understanding of drug activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.