ArticleNature communications2014
The full-length cell-cell fusogen EFF-1 is monomeric and upright on the membrane.
Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 48 citations in OpenAlex.
- Visualization of membrane-stabilized SorCS2Journal of structural biology: X · 2026Article
- GPX modulation promotes regenerative axonal fusion and functional recovery after injury through PSR-1 condensation.Nature communications · 2025Article
- Extracellular vesicles.Genetics · 2024Review
- Novel requirements for HAP2/GCS1-mediated gamete fusion iniScience · 2024Article
- Eukaryotic fertilization and gamete fusion at a glance.Journal of cell science · 2022Article
- Recent Advances in Bunyavirus Glycoprotein Research: Precursor Processing, Receptor Binding and Structure.Viruses · 2021Review
- DNA origami signposts for identifying proteins on cell membranes by electron cryotomography.Cell · 2021Article
- Evolutionarily related small viral fusogens hijack distinct but modular actin nucleation pathways to drive cell-cell fusion.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- HAP2-Mediated Gamete Fusion: Lessons From the World of Unicellular Eukaryotes.Frontiers in cell and developmental biology · 2021Review
- Fusexins, HAP2/GCS1 and Evolution of Gamete Fusion.Frontiers in cell and developmental biology · 2021Review
- Identification and Characteristics of Fusion Peptides Derived From Enveloped Viruses.Frontiers in chemistry · 2021Review
- The importance of the membrane for biophysical measurements.Nature chemical biology · 2020Review
- Article
- Variability in β-catenin pulse dynamics in a stochastic cell fate decision in C. elegans.Developmental biology · 2020Article
- Disruption of RAB-5 Increases EFF-1 Fusogen Availability at the Cell Surface and Promotes the Regenerative Axonal Fusion Capacity of the Neuron.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry.Science (New York, N.Y.) · 2018Article
- The AFF-1 exoplasmic fusogen is required for endocytic scission and seamless tube elongation.Nature communications · 2018Article
- WASP-Arp2/3-dependent actin polymerization influences fusogen localization during cell-cell fusion inBiology open · 2017Article
- Time to make the doughnuts: Building and shaping seamless tubes.Seminars in cell & developmental biology · 2017Review
- Native structure of a retroviral envelope protein and its conformational change upon interaction with the target cell.Journal of structural biology · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Fusogens are membrane proteins that remodel lipid bilayers to facilitate membrane merging. Although several fusogen ectodomain structures have been solved, structural information on full-length, natively membrane-anchored fusogens is scarce. Here we present the electron cryo microscopy three-dimensional reconstruction of the Caenorhabditis elegans epithelial fusion failure 1 (EFF-1) protein natively anchored in cell-derived membrane vesicles. This reveals a membrane protruding, asymmetric, elongated monomer. Flexible fitting of a protomer of the EFF-1 crystal structure, which is homologous to viral class-II fusion proteins, shows that EFF-1 has a hairpin monomeric conformation before fusion. These structural insights, when combined with our observations of membrane-merging intermediates between vesicles, enable us to propose a model for EFF-1 mediated fusion. This process, involving identical proteins on both membranes to be fused, follows a mechanism that shares features of SNARE-mediated fusion while using the structural building blocks of the unilaterally acting class-II viral fusion proteins.
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