ArticlePloS one2014
A novel antifungal is active against Candida albicans biofilms and inhibits mutagenic acetaldehyde production in vitro.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- Article
- A Novel Benzopyrone Derivative from Streptomyces chrestomyceticus ADP4 Inhibits Growth and Virulence Factors of Candida albicans.Current microbiology · 2025Article
- Identification and characterization of a novel decalin derivative with anti-Candida activity from Streptomyces chrestomyceticus strain ADP4.Archives of microbiology · 2024Article
- Article
- Candidiasis in breast cancer: Tumor progression or not?Iranian journal of basic medical sciences · 2024Review
- Two promisingFrontiers in pharmacology · 2024Article
- Selection of Ethanol Tolerant Strains ofbioRxiv : the preprint server for biology · 2023Article
- In Vitro Anti-Microorganisms · 2023Article
- Adhesion and Anti-Adhesion Abilities of Potentially Probiotic Lactic Acid Bacteria and Biofilm Eradication of Honeybee (Molecules (Basel, Switzerland) · 2022Article
- Lactobacillus rhamnosus colonisation antagonizes Candida albicans by forcing metabolic adaptations that compromise pathogenicity.Nature communications · 2022Article
- Some Important Metabolites Produced by Lactic Acid Bacteria Originated from Kimchi.Foods (Basel, Switzerland) · 2021Review
- Mechanisms of Candida Resistance to Antimycotics and Promising Ways to Overcome It: The Role of Probiotics.Probiotics and antimicrobial proteins · 2021Review
- Intestinal Infection ofFrontiers in microbiology · 2021Review
- A novel study on the inhibitory effect of marine macroalgal extracts on hyphal growth and biofilm formation of candidemia isolates.Scientific reports · 2020Article
- Global proteomic analysis deciphers the mechanism of action of plant derived oleic acid against Candida albicans virulence and biofilm formation.Scientific reports · 2020Article
- Alteration of Fermentative Metabolism Enhances Mucor circinelloides Virulence.Infection and immunity · 2020Article
- Local Acetaldehyde-An Essential Role in Alcohol-Related Upper Gastrointestinal Tract Carcinogenesis.Cancers · 2018Review
- Influence of culture media on biofilm formation by Candida species and response of sessile cells to antifungals and oxidative stress.BioMed research international · 2015Article
- DL-2-hydroxyisocaproic acid attenuates inflammatory responses in a murine Candida albicans biofilm model.Clinical and vaccine immunology : CVI · 2014Article
- A novel antifungal is active against Candida albicans biofilms and inhibits mutagenic acetaldehyde production in vitro.PloS one · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of C. albicans to form biofilms is a major virulence factor and a challenge for management. This is evident in biofilm-associated chronic oral-oesophageal candidosis, which has been shown to be potentially carcinogenic in vivo. We have previously shown that most Candida spp. can produce significant levels of mutagenic acetaldehyde (ACH). ACH is also an important mediator of candidal biofilm formation. We have also reported that D,L-2-hydroxyisocaproic acid (HICA) significantly inhibits planktonic growth of C. albicans. The aim of the present study was to investigate the effect of HICA on C. albicans biofilm formation and ACH production in vitro. Inhibition of biofilm formation by HICA, analogous control compounds or caspofungin was measured using XTT to measure biofilm metabolic activity and PicoGreen as a marker of biomass. Biofilms were visualised by scanning electron microscopy (SEM). ACH levels were measured by gas chromatography. Transcriptional changes in the genes involved in ACH metabolism were measured using RT-qPCR. The mean metabolic activity and biomass of all pre-grown (4, 24, 48 h) biofilms were significantly reduced after exposure to HICA (p<0.05) with the largest reductions seen at acidic pH. Caspofungin was mainly active against biofilms pre-grown for 4 h at neutral pH. Mutagenic levels (>40 µM) of ACH were detected in 24 and 48 h biofilms at both pHs. Interestingly, no ACH production was detected from D-glucose in the presence of HICA at acidic pH (p<0.05). Expression of genes responsible for ACH catabolism was up-regulated by HICA but down-regulated by caspofungin. SEM showed aberrant hyphae and collapsed hyphal structures during incubation with HICA at acidic pH. We conclude that HICA has potential as an antifungal agent with ability to inhibit C. albicans cell growth and biofilm formation. HICA also significantly reduces the mutagenic potential of C. albicans biofilms, which may be important when treating bacterial-fungal biofilm infections.
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