ArticleInfection and immunity2014
Plasmid CDS5 influences infectivity and virulence in a mouse model of Chlamydia trachomatis urogenital infection.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 37 citations in OpenAlex.
- Plasmid-encoded Pgp3 protein enhances the infectivity ofZhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2026Article
- Study Models forMicroorganisms · 2025Review
- Diverse animal models forFrontiers in microbiology · 2024Review
- The emerging complexity of Chlamydia trachomatis interactions with host cells as revealed by molecular genetic approaches.Current opinion in microbiology · 2023Review
- Chlamydia trachomatis plasmid-encoding Pgp3 protein induces secretion of distinct inflammatory signatures from HeLa cervical epithelial cells.BMC microbiology · 2023Article
- Virulence Protein Pgp3 Is Insufficient To Mediate Plasmid-Dependent Infectivity of Chlamydia trachomatis.Infection and immunity · 2023Article
- Plasmid-mediated virulence inFrontiers in cellular and infection microbiology · 2023Review
- Chlamydia Deficient in Plasmid-Encoded Glycoprotein 3 (pGP3) as an Attenuated Live Oral Vaccine.Infection and immunity · 2022Article
- Completing the Genome Sequence ofPathogens (Basel, Switzerland) · 2021Article
- A Nonsurgical Embryo Transfer Technique for Fresh and Cultured Blastocysts in Rats.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Article
- The Nature and Extent of Plasmid Variation inMicroorganisms · 2020Article
- Diversity in Chlamydial plasmids.PloS one · 2020Article
- The multiple functions of the numerousMicrobial cell (Graz, Austria) · 2019Review
- Chlamydia muridarum Induces Pathology in the Female Upper Genital Tract via Distinct Mechanisms.Infection and immunity · 2019Article
- The Structural Integrity of Plasmid-Encoded Pgp3 Is Essential for Induction of Hydrosalpinx byFrontiers in cellular and infection microbiology · 2019Article
- Chlamydia Spreading from the Genital Tract to the Gastrointestinal Tract - A Two-Hit Hypothesis.Trends in microbiology · 2018Review
- Article
- Genomic analyses of the Chlamydia trachomatis core genome show an association between chromosomal genome, plasmid type and disease.BMC genomics · 2018Article
- Plasmid Negative Regulation of CPAF Expression Is Pgp4 Independent and Restricted to InvasivemBio · 2018Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
The native plasmid of both Chlamydia muridarum and Chlamydia trachomatis has been shown to control virulence and infectivity in mice and in lower primates. We recently described the development of a plasmid-based genetic transformation protocol for Chlamydia trachomatis that for the first time provides a platform for the molecular dissection of the function of the chlamydial plasmid and its individual genes or coding sequences (CDS). In the present study, we transformed a plasmid-free lymphogranuloma venereum isolate of C. trachomatis, serovar L2, with either the original shuttle vector (pGFP::SW2) or a derivative of pGFP::SW2 carrying a deletion of the plasmid CDS5 gene (pCDS5KO). Female mice were inoculated with these strains either intravaginally or transcervically. We found that transformation of the plasmid-free isolate with the intact pGFP::SW2 vector significantly enhanced infectivity and induction of host inflammatory responses compared to the plasmid-free parental isolate. Transformation with pCDS5KO resulted in infection courses and inflammatory responses not significantly different from those observed in mice infected with the plasmid-free isolate. These results indicate a critical role of plasmid CDS5 in in vivo fitness and in induction of inflammatory responses. To our knowledge, these are the first in vivo observations ascribing infectivity and virulence to a specific plasmid gene.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.