ArticleNaunyn-Schmiedeberg's archives of pharmacology2014
Three commercial antibodies against α1-adrenergic receptor subtypes lack specificity in paraffin-embedded sections of murine tissues.
Article in Naunyn-Schmiedeberg's archives of pharmacology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Cardiac and Vascular αCells · 2020Pooled it
- αPharmaceuticals (Basel, Switzerland) · 2025Review
- Adrenoceptors in the Eye - Physiological and Pathophysiological Relevance.Handbook of experimental pharmacology · 2024Review
- β-Adrenoreceptors as Therapeutic Targets for Ocular Tumors and Other Eye Diseases-Historical Aspects and Nowadays Understanding.International journal of molecular sciences · 2023Review
- Radioligand Binding to Quantify Adrenergic Receptor Expression in the Heart.Current protocols · 2023Article
- Antidepressants Differentially Regulate Intracellular Signaling from α1-Adrenergic Receptor Subtypes In Vitro.International journal of molecular sciences · 2021Article
- Current Developments on the Role of αFrontiers in cell and developmental biology · 2021Review
- The Role of Adrenoceptors in the Retina.Cells · 2020Review
- Von Economo Neurons and Fork Cells: A Neurochemical Signature Linked to Monoaminergic Function.Cerebral cortex (New York, N.Y. : 1991) · 2018Article
- Cortical adrenoceptor expression, function and adaptation under conditions of cannabinoid receptor deletion.Experimental neurology · 2017Article
- Adrenergic Receptors in Individual Ventricular Myocytes: The Beta-1 and Alpha-1B Are in All Cells, the Alpha-1A Is in a Subpopulation, and the Beta-2 and Beta-3 Are Mostly Absent.Circulation research · 2017Article
- A comparison of phosphospecific affinity reagents reveals the utility of recombinant Forkhead-associated domains in recognizing phosphothreonine-containing peptides.New biotechnology · 2016Article
- Naunyn-Schmiedeberg's Archives of Pharmacology under new editorship: change and continuity.Naunyn-Schmiedeberg's archives of pharmacology · 2016Article
- Commercially available antibodies directed against α-adrenergic receptor subtypes and other G protein-coupled receptors with acceptable selectivity in flow cytometry experiments.Naunyn-Schmiedeberg's archives of pharmacology · 2016Article
- Nuclear compartmentalization of α1-adrenergic receptor signaling in adult cardiac myocytes.Journal of cardiovascular pharmacology · 2015Review
- Rat β₃-adrenoceptor protein expression: antibody validation and distribution in rat gastrointestinal and urogenital tissues.Naunyn-Schmiedeberg's archives of pharmacology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We tested the specificity of three commercially available antibodies (AB) against individual α1-adrenergic receptor subtypes (α1-ARST). We used these ABs to localize the α1-ARST proteins by immunohistochemistry in paraffin-embedded murine tissues. The specificity of the ABs was tested by comparing staining patterns in tissues from wild-type mice with those in corresponding tissues from mice with gene-targeted disruption of the respective α1-ARST, one of the most rigorous negative controls. None of the tested ABs proved to be specific for the indicated target antigen. We conclude that the tested ABs are unsuitable for immunohistochemistry in paraffin-embedded murine tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.