ArticlePloS one2014
Blood feeding and Plasmodium infection alters the miRNome of Anopheles stephensi.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- microRNA profiles and functions in mosquitoes.PLoS neglected tropical diseases · 2018Pooled it
- A brain-specific microRNA,Genes & development · 2026Article
- De Novo miRNAs fromInsects · 2024Article
- microRNA Expression Dynamics inInsects · 2023Article
- Small RNA sequencing of field Culex mosquitoes identifies patterns of viral infection and the mosquito immune response.Scientific reports · 2023Article
- Review
- Harnessing the Potential of miRNAs in Malaria Diagnostic and Prevention.Frontiers in cellular and infection microbiology · 2021Review
- Development of miRNA-Based Approaches to Explore the Interruption of Mosquito-Borne Disease Transmission.Frontiers in cellular and infection microbiology · 2021Review
- Improved reference genome of the arboviral vector Aedes albopictus.Genome biology · 2020Article
- Global Analysis of Small Non-Coding RNA Populations across Tissues in the Malaria Vector,Insects · 2020Article
- Review
- Small RNA-Seq Analysis Reveals miRNA Expression Dynamics Across Tissues in the Malaria Vector,G3 (Bethesda, Md.) · 2019Article
- MicroRNAs from saliva of anopheline mosquitoes mimic human endogenous miRNAs and may contribute to vector-host-pathogen interactions.Scientific reports · 2019Article
- Differential plasma microvesicle and brain profiles of microRNA in experimental cerebral malaria.Malaria journal · 2018Article
- Analysis of microRNA profile of Anopheles sinensis by deep sequencing and bioinformatic approaches.Parasites & vectors · 2018Article
- Evidence that microRNAs are part of the molecular toolkit regulating adult reproductive diapause in the mosquito, Culex pipiens.PloS one · 2018Article
- Association of microRNAs with Argonaute proteins in the malaria mosquito Anopheles gambiae after blood ingestion.Scientific reports · 2017Article
- Comparative expression profile of microRNAs in Anopheles anthropophagus midgut after blood-feeding and Plasmodium infection.Parasites & vectors · 2017Article
- Comparative genomics identifies male accessory gland proteins in fiveWellcome open research · 2017Article
- Tying malaria and microRNAs: from the biology to future diagnostic perspectives.Malaria journal · 2016Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Blood feeding is an integral process required for physiological functions and propagation of the malaria vector Anopheles. During blood feeding, presence of the malaria parasite, Plasmodium in the blood induces several host effector molecules including microRNAs which play important roles in the development and maturation of the parasite within the mosquito. The present study was undertaken to elucidate the dynamic expression of miRNAs during gonotrophic cycle and parasite development in Anopheles stephensi. Using next generation sequencing technology, we identified 126 miRNAs of which 17 were novel miRNAs. The miRNAs were further validated by northern hybridization and cloning. Blood feeding and parasitized blood feeding in the mosquitoes revealed regulation of 13 and 16 miRNAs respectively. Expression profiling of these miRNAs revealed that significant miRNAs were down-regulated upon parasitized blood feeding with a repertoire of miRNAs showing stage specific up-regulation. Expression profiles of significantly modulated miRNAs were further validated by real time PCR. Target prediction of regulated miRNAs revealed overlapping targeting by different miRNAs. These targets included several metabolic pathways including metabolic, redox homeostasis and protein processing machinery components. Our analysis revealed tight regulation of specific miRNAs post blood feeding and parasite infection in An. stephensi. Such regulated expression suggests possible role of these miRNAs during gonotrophic cycle in mosquito. Another set of miRNAs were also significantly regulated at 42 h and 5 days post infection indicating parasite stage-specific role of host miRNAs. This study will result in better understanding of the role of miRNAs during gonotrophic cycle and parasite development in mosquito and can probably facilitate in devising novel malaria control strategies at vector level.
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