ArticleJournal of fluorescence2014
Testing fluorescence lifetime standards using two-photon excitation and time-domain instrumentation: rhodamine B, coumarin 6 and lucifer yellow.
Article in Journal of fluorescence, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 161 citations in OpenAlex.
- Development of solid-state fluorescence lifetime standards for clinical applications using dyed epoxy resins.Journal of biomedical optics · 2026Article
- Reversible, Chemically Gated FRET via Ligand-Activated Acceptors.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Lifetime Standard for Fluorescence Lifetime Imaging Ophthalmoscopy.Translational vision science & technology · 2026Article
- Denoising of fluorescence lifetime imaging data via principal component analysis.Scientific reports · 2025Article
- Laser-emission vibrational microscopy of microdroplet arrays for high-throughput screening of hyperlipidemia.Light, science & applications · 2025Article
- Photoinduced chromophore dissociation resulting in aggregation-induced red fluorescence.Communications biology · 2025Article
- An update on recent advances in fluorescent materials for fluorescence molecular imaging: a review.RSC advances · 2025Review
- The development of mitochondria-targeted quercetin for rescuing Sertoli cells from oxidative stress.Research in pharmaceutical sciences · 2025Article
- High-throughput fluorescence lifetime imaging flow cytometry.Nature communications · 2024Article
- Applications of machine learning in time-domain fluorescence lifetime imaging: a review.Methods and applications in fluorescence · 2024Review
- Article
- Biomolecular condensates modulate membrane lipid packing and hydration.Nature communications · 2023Article
- Photocatalytic and Photothermal Antimicrobial Mussel-Inspired Nanocomposites for Biomedical Applications.International journal of molecular sciences · 2023Article
- Light-sheet autofluorescence lifetime imaging with a single-photon avalanche diode array.Journal of biomedical optics · 2023Article
- Light sheet autofluorescence lifetime imaging with a single photon avalanche diode array.bioRxiv : the preprint server for biology · 2023Article
- Label-free spatially maintained measurements of metabolic phenotypes in cells.Frontiers in bioengineering and biotechnology · 2023Article
- Absolute excited state molecular geometries revealed by resonance Raman signals.Nature communications · 2022Article
- Comprehensive Investigation of Parameters Influencing Fluorescence Lifetime Imaging Microscopy in Frequency- and Time-Domain Illustrated by Phasor Plot Analysis.International journal of molecular sciences · 2022Article
- Three-dimensional imaging of biological cells using surface plasmon coupled emission.Journal of biomedical optics · 2022Article
- Simple and Robust Deep Learning Approach for Fast Fluorescence Lifetime Imaging.Sensors (Basel, Switzerland) · 2022Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Having good information about fluorescence lifetime standards is essential for anyone performing lifetime experiments. Using lifetime standards in fluorescence spectroscopy is often regarded as a straightforward process, however, many earlier reports are limited in terms of lifetime concentration dependency, solvents and other technical aspects. We have investigated the suitability of the fluorescent dyes rhodamine B, coumarin 6, and lucifer yellow as lifetime standards, especially to be used with two-photon excitation measurements in the time-domain. We measured absorption and emission spectra for the fluorophores to determine which wavelengths we should use for the excitation and an appropriate detector range. We also measured lifetimes for different concentrations, ranging from 10(-2)- 10(-6) M, in both water, ethanol and methanol solutions. We observed that rhodamine B lifetimes depend strongly on concentration. Coumarin 6 provided the most stable lifetimes, with a negligible dependency on concentration and solvent. Lucifer yellow lifetimes were also found to depend little with concentration. Finally, we found that a mix of two fluorophores (rhodamine B/coumarin 6, rhodamine B/lucifer yellow, and coumarin 6/lucifer yellow) all yielded very similar lifetimes from a double-exponential decay as the separate lifetimes measured from a single-exponential decay. All lifetime measurements were made using two-photon excitation and obtaining lifetime data in the time-domain using time-correlated single-photon counting.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.