ArticleMolecular & cellular proteomics : MCP2014
Dissecting the subcellular compartmentation of proteins and metabolites in arabidopsis leaves using non-aqueous fractionation.
Article in Molecular & cellular proteomics : MCP, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Chorismate and isochorismate turnover reveal hidden dynamics of aromatic metabolism in Arabidopsis leaves.The Plant cell · 2026Article
- The sucrose-trehalose 6-phosphate nexus is conserved in flowering plants with different phloem loading and carbon storage strategies.Journal of experimental botany · 2026Article
- Hypoxia induces a glycolytic complex in intestinal epithelial cells independent of HIF-1-driven glycolytic gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Retrograde signaling in plants: A critical review focusing on the GUN pathway and beyond.Plant communications · 2023Review
- "Omics" insights into plastid behavior toward improved carotenoid accumulation.Frontiers in plant science · 2022Review
- Regulatory Modules of Metabolites and Protein Phosphorylation in Arabidopsis Genotypes With Altered Sucrose Allocation.Frontiers in plant science · 2022Article
- Mapping the plant proteome: tools for surveying coordinating pathways.Emerging topics in life sciences · 2021Review
- Analysis of Nucleosides and Nucleotides in Plants: An Update on Sample Preparation and LC-MS Techniques.Cells · 2021Review
- Tracing metabolic flux through time and space with isotope labeling experiments.Current opinion in biotechnology · 2020Review
- Rapid Affinity Purification of Tagged Plant Mitochondria (Mito-AP) for Metabolome and Proteome Analyses.Plant physiology · 2020Article
- Resolving subcellular plant metabolism.The Plant journal : for cell and molecular biology · 2019Article
- Non-aqueous Fractionation (NAF) for Metabolite Analysis in Subcellular Compartments ofBio-protocol · 2019Article
- Article
- The Plastidic Sugar Transporter pSuT Influences Flowering and Affects Cold Responses.Plant physiology · 2019Article
- The challenge and potential of photosynthesis: unique considerations for metabolic flux measurements in photosynthetic microorganisms.Biotechnology letters · 2019Review
- Non-aqueous fractionation revealed changing subcellular metabolite distribution during apple fruit development.Horticulture research · 2019Article
- Subcellular Phenotyping: Using Proteomics to Quantitatively Link Subcellular Leaf Protein and Organelle Distribution Analyses ofFrontiers in plant science · 2019Article
- Advances in metabolic flux analysis toward genome-scale profiling of higher organisms.Bioscience reports · 2018Review
- Enzymatic complexes across scales.Essays in biochemistry · 2018Review
- Subcellular metabolomics: the choice of method depends on the aim of the study.Journal of experimental botany · 2017Article
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11 authors.
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Abstract
Non-aqueous fractionation is a technique for the enrichment of different subcellular compartments derived from lyophilized material. It was developed to study the subcellular distribution of metabolites. Here we analyzed the distribution of about 1,000 proteins and 70 metabolites, including 22 phosphorylated intermediates in wild-type Arabidopsis rosette leaves, using non-aqueous gradients divided into 12 fractions. Good separation of plastidial, cytosolic, and vacuolar metabolites and proteins was achieved, but cytosolic, mitochondrial, and peroxisomal proteins clustered together. There was considerable heterogeneity in the fractional distribution of transcription factors, ribosomal proteins, and subunits of the vacuolar-ATPase, indicating diverse compartmental location. Within the plastid, sub-organellar separation of thylakoids and stromal proteins was observed. Metabolites from the Calvin-Benson cycle, photorespiration, starch and sucrose synthesis, glycolysis, and the tricarboxylic acid cycle grouped with their associated proteins of the respective compartment. Non-aqueous fractionation thus proved to be a powerful method for the study of the organellar, and in some cases sub-organellar, distribution of proteins and their association with metabolites. It remains the technique of choice for the assignment of subcellular location to metabolites in intact plant tissues, and thus the technique of choice for doing combined metabolite-protein analysis on a single tissue sample.
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