ArticleMolecular and cellular biology2014
Histidine methylation of yeast ribosomal protein Rpl3p is required for proper 60S subunit assembly.
Article in Molecular and cellular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Histidine N1-position-specific methyltransferase CARNMT1 targets C3H zinc finger proteins and modulates RNA metabolism.Genes & development · 2023Article
- A seven-transmembrane methyltransferase catalysing N-terminal histidine methylation of lytic polysaccharide monooxygenases.Nature communications · 2023Article
- The protein methylation network in yeast: A landmark in completeness for a eukaryotic post-translational modification.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Molecular basis for protein histidine N1-specific methylation of the "His-x-His" motifs by METTL9.Cell insight · 2023Article
- Article
- METTL9 mediated N1-histidine methylation of zinc transporters is required for tumor growth.Protein & cell · 2021Review
- Regulation of ribosomal protein genes: An ordered anarchy.Wiley interdisciplinary reviews. RNA · 2021Review
- Human METTL18 is a histidine-specific methyltransferase that targets RPL3 and affects ribosome biogenesis and function.Nucleic acids research · 2021Article
- Protein Methylation and Translation: Role of Lysine Modification on the Function of Yeast Elongation Factor 1A.Biochemistry · 2019Article
- SETD3 protein is the actin-specific histidineeLife · 2018Article
- The ribosome: A hot spot for the identification of new types of protein methyltransferases.The Journal of biological chemistry · 2018Article
- Metabolic footprint analysis of metabolites that discriminate single and mixed yeast cultures at two key time-points during mixed culture alcoholic fermentations.Metabolomics : Official journal of the Metabolomic Society · 2018Article
- Molecular basis for histidine N1 position-specific methylation by CARNMT1.Cell research · 2018Article
- Determining the Mitochondrial Methyl Proteome in Saccharomyces cerevisiae using Heavy Methyl SILAC.Journal of proteome research · 2016Article
- Splicing-Mediated Autoregulation Modulates Rpl22p Expression in Saccharomyces cerevisiae.PLoS genetics · 2016Article
- Methylation of yeast ribosomal protein Rpl3 promotes translational elongation fidelity.RNA (New York, N.Y.) · 2016Article
- Ribosomal protein methyltransferases in the yeast Saccharomyces cerevisiae: Roles in ribosome biogenesis and translation.Biochemical and biophysical research communications · 2016Article
- Yeast Coq9 controls deamination of coenzyme Q intermediates that derive from para-aminobenzoic acid.Biochimica et biophysica acta · 2015Article
- Lysine Methylation of the Valosin-Containing Protein (VCP) Is Dispensable for Development and Survival of Mice.PloS one · 2015Article
- A new type of protein lysine methyltransferase trimethylates Lys-79 of elongation factor 1A.Biochemical and biophysical research communications · 2014Article
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6 authors.
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Abstract
Histidine protein methylation is an unusual posttranslational modification. In the yeast Saccharomyces cerevisiae, the large ribosomal subunit protein Rpl3p is methylated at histidine 243, a residue that contacts the 25S rRNA near the P site. Rpl3p methylation is dependent upon the presence of Hpm1p, a candidate seven-beta-strand methyltransferase. In this study, we elucidated the biological activities of Hpm1p in vitro and in vivo. Amino acid analyses reveal that Hpm1p is responsible for all of the detectable protein histidine methylation in yeast. The modification is found on a polypeptide corresponding to the size of Rpl3p in ribosomes and in a nucleus-containing organelle fraction but was not detected in proteins of the ribosome-free cytosol fraction. In vitro assays demonstrate that Hpm1p has methyltransferase activity on ribosome-associated but not free Rpl3p, suggesting that its activity depends on interactions with ribosomal components. hpm1 null cells are defective in early rRNA processing, resulting in a deficiency of 60S subunits and translation initiation defects that are exacerbated in minimal medium. Cells lacking Hpm1p are resistant to cycloheximide and verrucarin A and have decreased translational fidelity. We propose that Hpm1p plays a role in the orchestration of the early assembly of the large ribosomal subunit and in faithful protein production.
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