ArticleGut pathogens2014
A novel enzyme-linked immunosorbent assay for detection of Escherichia coli O157:H7 using immunomagnetic and beacon gold nanoparticles.
Article in Gut pathogens, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 95 citations in OpenAlex.
- Emerging Nanomaterials-Based Biosensors for the Clinical and Pathological Detection of E. Coli O157:H7.Current microbiology · 2026Review
- Design of ELISA-based diagnostic system for detection of enterohaemorrhagicIranian journal of microbiology · 2025Article
- Development and Characterization of a Polyvalent Polyclonal Antibody as a Common Capture Antibody for the Detection of Enterotoxigenic Escherichia coli in a Sandwich ELISA.Current microbiology · 2025Article
- Review
- Rapid, Selective, and Ultra-Sensitive Field Effect Transistor-Based Detection ofMaterials (Basel, Switzerland) · 2024Article
- Rapid detection of pathogenicFrontiers in microbiology · 2024Article
- Ultrasensitive and Rapid Visual Detection ofBiosensors · 2023Article
- Bacteriophage-based nano-biosensors for the fast impedimetric determination of pathogens in food samples.Scientific reports · 2023Article
- Simultaneous Detection ofBiosensors · 2022Article
- Transcription Factor-Based Biosensors for Detecting Pathogens.Biosensors · 2022Review
- Electrochemical Detection of Waterborne Bacteria Using Bi-Functional Magnetic Nanoparticle Conjugates.Biosensors · 2022Article
- Electrochemical Immuno- and Aptamer-Based Assays for Bacteria: Pros and Cons over Traditional Detection Schemes.Sensors (Basel, Switzerland) · 2020Review
- Screen-Printed Electrodes (SPE) for In Vitro Diagnostic Purpose.Diagnostics (Basel, Switzerland) · 2020Review
- Review
- Power of Scanning Electron Microscopy and Energy Dispersive X-Ray Analysis in Rapid Microbial Detection and Identification at the Single Cell Level.Scientific reports · 2020Article
- Rapid detection of Escherichia coli in beverages using genetically engineered bacteriophage T7.AMB Express · 2019Article
- A Fluidic Device for Immunomagnetic Separation of Foodborne Bacteria Using Self-Assembled Magnetic Nanoparticle Chains.Micromachines · 2018Article
- Electrochemical Detection ofSensors (Basel, Switzerland) · 2018Article
- Comparison of four functionalization methods of gold nanoparticles for enhancing the enzyme-linked immunosorbent assay (ELISA).Beilstein journal of nanotechnology · 2017Article
- Bioinspired Synthesis of All-in-One Organic-Inorganic Hybrid Nanoflowers Combined with a Handheld pH Meter for On-Site Detection of Food Pathogen.Small (Weinheim an der Bergstrasse, Germany) · 2016Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This paper presents a functional nanoparticle-enhanced enzyme-linked immunosorbent assay (FNP-ELISA) for detection of enterohemorrhagic Escherichia coli (EHEC) O157:H7. Immunomagnetic nanoparticles (IMMPs) conjugated with monoclonal anti-O157:H7 antibody were used to capture E. coli O157:H7. Beacon gold nanoparticles (B-GNPs) coated with polyclonal anti-O157:H7 and biotin single-stranded DNA (B-DNA) were then subjective to immunoreaction with E. coli O157:H7, which was followed by streptavidin-horseradish peroxidase (Strep-HRP) conjugated with B-GNPs based on a biotin-avidin system. The solutions containing E. coli O157:H7, IMMPs, B-GNPs, and Strep-HRP were collected for detecting color change. The signal was significantly amplified with detection limits of 68 CFU mL(-1) in PBS and 6.8 × 10(2) to 6.8 × 10(3) CFU mL(-1) in the food samples. The FNP-ELISA method developed in this study was two orders of magnitude more sensitive than immunomagnetic separation ELISA (IMS-ELISA) and four orders of magnitude more sensitive than C-ELISA. The entire detection process of E. coli O157:H7 lasted only 3 h, and thus FNP-ELISA is considered as a time-saving method.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.