ArticleThe Journal of cell biology2014
CENP-I and Aurora B act as a molecular switch that ties RZZ/Mad1 recruitment to kinetochore attachment status.
Article in The Journal of cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 56 citations in OpenAlex.
- The dynamic role of nucleoprotein SHCBP1 in the cancer cell cycle and its potential as a synergistic target for DNA-damaging agents in cancer therapy.Cell communication and signaling : CCS · 2024Article
- Aurora A phosphorylates Ndel1 to reduce the levels of Mad1 and NuMA at spindle poles.Molecular biology of the cell · 2023Article
- Pan-Cancer Analysis Reveals CENPI as a Potential Biomarker and Therapeutic Target in Adrenocortical Carcinoma.Journal of inflammation research · 2023Article
- Aurora B activity is promoted by cooperation between discrete localization sites in budding yeast.Molecular biology of the cell · 2022Article
- Structure of the RZZ complex and molecular basis of Spindly-driven corona assembly at human kinetochores.The EMBO journal · 2022Article
- The Role of Mitotic Kinases and the RZZ Complex in Kinetochore-Microtubule Attachments: Doing the Right Link.Frontiers in cell and developmental biology · 2022Review
- Increased Aurora B expression reduces substrate phosphorylation and induces chromosomal instability.Frontiers in cell and developmental biology · 2022Article
- Identification of 22 susceptibility loci associated with testicular germ cell tumors.Nature communications · 2021Article
- Mycoplasma hyopneumoniae J elicits an antioxidant response and decreases the expression of ciliary genes in infected swine epithelial cells.Scientific reports · 2020Article
- RZZ-SPINDLY-DYNEIN: you got to keep 'em separated.Cell cycle (Georgetown, Tex.) · 2020Review
- Spindle Architectural Features Must Be Considered Along With Cell Size to Explain the Timing of Mitotic Checkpoint Silencing.Frontiers in physiology · 2020Article
- Mammalian kinetochores count attached microtubules in a sensitive and switch-like manner.The Journal of cell biology · 2019Article
- ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.Nucleic acids research · 2019Article
- CRISPR knockout screening identifies combinatorial drug targets in pancreatic cancer and models cellular drug response.Nature communications · 2018Article
- The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases.The Journal of biological chemistry · 2018Article
- The ESCRT protein Chmp4c regulates mitotic spindle checkpoint signaling.The Journal of cell biology · 2018Article
- Article
- MAD1: Kinetochore Receptors and Catalytic Mechanisms.Frontiers in cell and developmental biology · 2018Review
- DNA damage induces a kinetochore-based ATM/ATR-independent SAC arrest unique to the first meiotic division in mouse oocytes.Development (Cambridge, England) · 2017Article
- Distinct Roles of the Chromosomal Passenger Complex in the Detection of and Response to Errors in Kinetochore-Microtubule Attachment.Developmental cell · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The RZZ (Rod, ZW10, and Zwilch) complex and Mad1 proteins tightly associate with kinetochores to generate the spindle checkpoint signal, but they are released when a kinetochore forms mature microtubule attachments. Here we demonstrate that the centromere protein CENP-I is required to generate a stable association of RZZ and Mad1 with kinetochores. CENP-I also inhibits their removal by dynein stripping. This regulation of Mad1 and RZZ dissociation functions independently of Aurora B, which regulates their association. We show that the microtubule status of each kinetochore independently dictates the recruitment of Aurora B kinase, kinase activity on a kinetochore substrate, and loading of spindle checkpoint proteins. This dynamic regulation of Mad1 association by Aurora B is only uncovered when CENP-I is depleted, consistent with our finding that CENP-I inhibits the dissociation of Mad1. We conclude that the dual activities of Aurora B and CENP-I generate a molecular switch that maintains a robust spindle checkpoint signal at prometaphase kinetochores until they attain mature attachments to microtubules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.