Trial reportEuropean journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology2014
Real-time intraoperative detection of breast cancer using near-infrared fluorescence imaging and Methylene Blue.
Trial report in European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers, 4 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
71 citing papers in PubMed, 4 syntheses or guidelines pooled it.
- Diagnostic accuracy and surgical outcomes of fluorescence-guided surgery in breast cancer: a systematic review and meta-analysis.World journal of surgical oncology · 2026Pooled it
- Fluorescence imaging for real-time detection of breast cancer tumors using IV injection of indocyanine green with non-conventional imaging: a systematic review of preclinical and clinical studies of perioperative imaging technologies.Breast cancer research and treatment · 2024Pooled it
- Fluorescence guided surgery imaging systems for breast cancer identification: a systematic review.Journal of biomedical optics · 2024Pooled it
- Pooled it
- Intraoperative imaging of folate receptor alpha positive ovarian and breast cancer using the tumor specific agent EC17.Oncotarget · 2016Trial
- Real-time Imaging of the Resection Bed Using a Handheld Probe to Reduce Incidence of Microscopic Positive Margins in Cancer Surgery.Cancer research · 2015Trial
- Trial
- Fluorescence Imaging of Cancer.Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer · 2026Review
- Advances in Imaging of Traumatic Nerve Injuries.The Journal of the American Academy of Orthopaedic Surgeons · 2025Review
- L-ICG as an optical agent to improve intraoperative margin detection in breast-conserving surgery: a prospective study.Breast cancer research and treatment · 2025Article
- Hyperspectral dark-field microscopy of human breast lumpectomy samples for tumor margin detection in breast-conserving surgery.Journal of biomedical optics · 2024Article
- The optimization and application of photodynamic diagnosis and autofluorescence imaging in tumor diagnosis and guided surgery: current status and future prospects.Frontiers in oncology · 2024Review
- Fluorescent Probes for Imaging in Humans: Where Are We Now?ACS nano · 2023Review
- Evaluating Receptor-Specific Fresh Specimen Staining for Tumor Margin Detection in Clinical Breast Specimens.Molecular imaging and biology · 2023Article
- High-Frequency (30 MHz-6 GHz) Breast Tissue Characterization Stabilized by Suction Force for Intraoperative Tumor Margin Assessment.Diagnostics (Basel, Switzerland) · 2023Article
- Near-infrared fluorophores methylene blue for targeted imaging of the stomach in intraoperative navigation.Frontiers in bioengineering and biotechnology · 2023Article
- Single Camera-Based Dual-Channel Near-Infrared Fluorescence Imaging system.Sensors (Basel, Switzerland) · 2022Article
- Drug Molecular Immobilization and Photofunctionalization of Calcium Phosphates for Exploring Theranostic Functions.Molecules (Basel, Switzerland) · 2022Review
- Quantifying the Photochemical Damage Potential of Contrast-Enhanced Fluorescence Imaging Products: Singlet Oxygen Production.Photochemistry and photobiology · 2022Article
- A clinically relevant formulation for direct administration of nerve specific fluorophores to mitigate iatrogenic nerve injury.Biomaterials · 2022Article
11 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors.
Funding
Abstract
backgroundDespite recent developments in preoperative breast cancer imaging, intraoperative localization of tumor tissue can be challenging, resulting in tumor-positive resection margins during breast conserving surgery. Based on certain physicochemical similarities between Technetium((99m)Tc)-sestamibi (MIBI), an SPECT radiodiagnostic with a sensitivity of 83-90% to detect breast cancer preoperatively, and the near-infrared (NIR) fluorophore Methylene Blue (MB), we hypothesized that MB might detect breast cancer intraoperatively using NIR fluorescence imaging.
methodsTwenty-four patients with breast cancer, planned for surgical resection, were included. Patients were divided in 2 administration groups, which differed with respect to the timing of MB administration. N = 12 patients per group were administered 1.0 mg/kg MB intravenously either immediately or 3 h before surgery. The mini-FLARE imaging system was used to identify the NIR fluorescent signal during surgery and on post-resected specimens transferred to the pathology department. Results were confirmed by NIR fluorescence microscopy.
results20/24 (83%) of breast tumors (carcinoma in N = 21 and ductal carcinoma in situ in N = 3) were identified in the resected specimen using NIR fluorescence imaging. Patients with non-detectable tumors were significantly older. No significant relation to receptor status or tumor grade was seen. Overall tumor-to-background ratio (TBR) was 2.4 ± 0.8. There was no significant difference between TBR and background signal between administration groups. In 2/4 patients with positive resection margins, breast cancer tissue identified in the wound bed during surgery would have changed surgical management. Histology confirmed the concordance of fluorescence signal and tumor tissue.
conclusionsThis feasibility study demonstrated an overall breast cancer identification rate using MB of 83%, with real-time intraoperative guidance having the potential to alter patient management.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.