ArticleMethods in enzymology2014
Use of chemical probes to detect mitochondrial ROS by flow cytometry and spectrofluorometry.
Article in Methods in enzymology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 9 citations in OpenAlex.
- Development of an adverse outcome pathway network for nephrotoxicity.Archives of toxicology · 2024Article
- Succinate dehydrogenase inhibitor dimethyl malonate alleviates LPS/d-galactosamine-induced acute hepatic damage in mice.Innate immunity · 2019Article
- Regulating intracellular ROS signal by a dual pH/reducing-responsive nanogels system promotes tumor cell apoptosis.International journal of nanomedicine · 2019Article
- The Type 1 Diabetes-Resistance LocusJournal of immunology (Baltimore, Md. : 1950) · 2017Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Aerobic respiration is a major source of energy in eukaryotic cells. In this setting, ATP production by the mitochondrial respiratory chain relies on the availability of NADH and FADH2 to donate protons and electrons. The flux of electrons down the electron transport chain, based on a series of oxidation-reduction reactions, releases energy that allow for the transport of H(+) ions across the inner mitochondrial membrane. The resulting proton-motive force is employed to drive ATP synthesis, while the final acceptor of the electrons flowing through the respiratory chain if molecular oxygen. A side effect of this process is the generation of reactive oxygen species (ROS). While mitochondrial ROS (mtROS) production has been linked to many pathological conditions (i.e., aging and tumorigenesis), recent evidence suggests that multiple cells, including malignant cells, employ these by-products of energy production as signals to control various cellular processes. Here, we describe protocols to use chemical probes for measuring mtROS production in intact cells by flow cytometry and spectrofluorometry.
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Registered trials
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