ArticleThe Plant journal : for cell and molecular biology2014
RNA-directed DNA methylation requires stepwise binding of silencing factors to long non-coding RNA.
Article in The Plant journal : for cell and molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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Who cites it
49 citing papers in PubMed, 87 citations in OpenAlex.
- Transcriptome analysis reveals long noncoding RNAs involved in the tuber anthocyanin synthesis of potato (Solanum tuberosum L.).BMC plant biology · 2026Article
- Gene Inactivation in Transgenic Plants-A Unique Model for Studying Epigenetic Regulation of Gene Expression.Plants (Basel, Switzerland) · 2026Review
- DNA methylation in plant heterosis: mechanisms and prospects.Functional & integrative genomics · 2025Review
- Biological Insights and Recent Advances in Plant Long Non-Coding RNA.International journal of molecular sciences · 2024Review
- Mathematical model of RNA-directed DNA methylation predicts tuning of negative feedback required for stable maintenance.Open biology · 2024Article
- Effects of Differentially Methylated CpG Sites in Enhancer and Promoter Regions on the Chromatin Structures of Target LncRNAs in Breast Cancer.International journal of molecular sciences · 2024Article
- Transcriptomic and epigenetic responses shed light on soybean resistance to Phytophthora sansomeana.The plant genome · 2024Article
- Article
- DDM1-Mediated TE Silencing in Plants.Plants (Basel, Switzerland) · 2023Review
- Factor of DNA methylation 1 affects woodland strawberry plant stature and organ size via DNA methylation.Plant physiology · 2023Article
- BPM1 regulates RdDM-mediated DNA methylation via a cullin 3 independent mechanism.Plant cell reports · 2022Article
- DNA hypermethylation promotes the flowering of orchardgrass during vernalization.Plant physiology · 2022Article
- Ovule siRNAs methylate protein-coding genes in trans.The Plant cell · 2022Article
- NODULIN HOMEOBOX is required for heterochromatin homeostasis in Arabidopsis.Nature communications · 2022Article
- Deciphering the tRNA-derived small RNAs: origin, development, and future.Cell death & disease · 2021Review
- Reinforcement of transcriptional silencing by a positive feedback between DNA methylation and non-coding transcription.Nucleic acids research · 2021Article
- Functions of long non-coding RNA inPlant signaling & behavior · 2021Review
- Arabidopsis MORC proteins function in the efficient establishment of RNA directed DNA methylation.Nature communications · 2021Article
- Identification of the Complex Interplay Between Nematode-Related lncRNAs and Their Target Genes inFrontiers in plant science · 2021Article
- Broad noncoding transcription suggests genome surveillance by RNA polymerase V.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Ribonucleic acid-mediated transcriptional gene silencing (known as RNA-directed DNA methylation, or RdDM, in Arabidopsis thaliana) is important for influencing gene expression and the inhibition of transposons by the deposition of repressive chromatin marks such as histone modifications and DNA methylation. A key event in de novo methylation of DNA by RdDM is the production of long non-coding RNA (lncRNA) by RNA polymerase V (Pol V). Little is known about the events that connect Pol V transcription to the establishment of repressive chromatin modifications. Using RNA immunoprecipitation, we elucidated the order of events downstream of lncRNA production and discovered interdependency between lncRNA-associated proteins. We found that the effector protein ARGONAUTE4 (AGO4) binds lncRNA independent of the RNA-binding protein INVOLVED IN DE NOVO2 (IDN2). In contrast, IDN2 binds lncRNA in an AGO4-dependent manner. We further found that the de novo DNA methyltransferase DOMAINS REARRANGED METHYLTRANSFERASE2 (DRM2) also associates with lncRNA produced by Pol V and that this event depends on AGO4 and IDN2. We propose a model where the silencing proteins AGO4, IDN2 and DRM2 bind to lncRNA in a stepwise manner, resulting in DNA methylation of RdDM target loci.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.