ArticleACS nano2014
Nanopore-based conformational analysis of a viral RNA drug target.
Article in ACS nano, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Single molecule nanopore counting assay targeting small extracellular vesicle cargo for non-invasive monitoring of cerebral organoid development and health.Scientific reports · 2025Article
- Supramolecular Interactions Modulate RNA:DNA Folding Observed via Nanopore Sensing.Angewandte Chemie (International ed. in English) · 2025Article
- Angular-Inertia Regulated Stable and Nanoscale Sensing of Single Molecules Using Nanopore-In-A-Tube.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Article
- In-tube micro-pyramidal silicon nanopore for inertial-kinetic sensing of single molecules.Nature communications · 2024Article
- Label-free and amplification-free viral RNA quantification from primate biofluids using a trapping-assisted optofluidic nanopore platform.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Real-time label-free detection of dynamic aptamer-small molecule interactions using a nanopore nucleic acid conformational sensor.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices.Micromachines · 2022Review
- Optical trapping assisted label-free and amplification-free detection of SARS-CoV-2 RNAs with an optofluidic nanopore sensor.Biosensors & bioelectronics · 2021Article
- Recent advances in biological nanopores for nanopore sequencing, sensing and comparison of functional variations in MspA mutants.RSC advances · 2021Review
- Recent advances in integrated solid-state nanopore sensors.Lab on a chip · 2021Review
- Structural-profiling of low molecular weight RNAs by nanopore trapping/translocation using Mycobacterium smegmatis porin A.Nature communications · 2021Article
- Synthetic heparan sulfate standards and machine learning facilitate the development of solid-state nanopore analysis.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Ribosome Fingerprinting with a Solid-State Nanopore.ACS sensors · 2020Article
- Microfluidic Systems Applied in Solid-State Nanopore Sensors.Micromachines · 2020Review
- Construction of RNA nanotubes.Nano research · 2019Article
- Nanopore electric snapshots of an RNA tertiary folding pathway.Nature communications · 2017Article
- Label-free nanopore single-molecule measurement of trypsin activity.ACS sensors · 2016Article
- Intrinsic and membrane-facilitated α-synuclein oligomerization revealed by label-free detection through solid-state nanopores.Scientific reports · 2016Article
- Electrophoretic Deformation of Individual Transfer RNA Molecules Reveals Their Identity.Nano letters · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Nanopores are single-molecule sensors that show exceptional promise as a biomolecular analysis tool by enabling label-free detection of small amounts of sample. In this paper, we demonstrate that nanopores are capable of detecting the conformation of an antiviral RNA drug target. The hepatitis C virus uses an internal ribosome entry site (IRES) motif in order to initiate translation by docking to ribosomes in its host cell. The IRES is therefore a viable and important drug target. Drug-induced changes to the conformation of the HCV IRES motif, from a bent to a straight conformation, have been shown to inhibit HCV replication. However, there is presently no straightforward method to analyze the effect of candidate small-molecule drugs on the RNA conformation. In this paper, we show that RNA translocation dynamics through a 3 nm diameter nanopore is conformation-sensitive by demonstrating a difference in transport times between bent and straight conformations of a short viral RNA motif. Detection is possible because bent RNA is stalled in the 3 nm pore, resulting in longer molecular dwell times than straight RNA. Control experiments show that binding of a weaker drug does not produce a conformational change, as consistent with independent fluorescence measurements. Nanopore measurements of RNA conformation can thus be useful for probing the structure of various RNA motifs, as well as structural changes to the RNA upon small-molecule binding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.