ArticleThe EMBO journal2014
Chromatin regulates DNA torsional energy via topoisomerase II-mediated relaxation of positive supercoils.
Article in The EMBO journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Electrophoretic Analysis of DNA Supercoiling Activities.Methods in molecular biology (Clifton, N.J.) · 2025Article
- An extrinsic motor directs chromatin loop formation by cohesin.The EMBO journal · 2024Article
- Chromatinization modulates topoisomerase II processivity.Nature communications · 2023Article
- Chromatinization Modulates Topoisomerase II Processivity.bioRxiv : the preprint server for biology · 2023Article
- Mechanical determinants of chromatin topology and gene expression.Nucleus (Austin, Tex.) · 2022Review
- Negative supercoils regulate meiotic crossover patterns in budding yeast.Nucleic acids research · 2022Article
- The roles of HMGB1-produced DNA gaps in DNA protection and aging biomarker reversal.FASEB bioAdvances · 2022Article
- A Novel Copper(II) Indenoisoquinoline Complex Inhibits Topoisomerase I, Induces G2 Phase Arrest, and Autophagy in Three Adenocarcinomas.Frontiers in oncology · 2022Article
- Transcription-mediated supercoiling regulates genome folding and loop formation.Molecular cell · 2021Article
- DNA-Topology Simplification by Topoisomerases.Molecules (Basel, Switzerland) · 2021Review
- Topoisomerase IIα represses transcription by enforcing promoter-proximal pausing.Cell reports · 2021Article
- Chromatin Architectural Factors as Safeguards against Excessive Supercoiling during DNA Replication.International journal of molecular sciences · 2020Review
- ALU non-B-DNA conformations, flipons, binary codes and evolution.Royal Society open science · 2020Review
- Weak interactions in higher-order chromatin organization.Nucleic acids research · 2020Review
- H3K4 methylation at active genes mitigates transcription-replication conflicts during replication stress.Nature communications · 2020Article
- Article
- The Texture of Chromatin.Cell · 2019Article
- Transcriptional supercoiling boosts topoisomerase II-mediated knotting of intracellular DNA.Nucleic acids research · 2019Article
- Are TADs supercoiled?Nucleic acids research · 2019Review
- Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint.PloS one · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic topoisomerases I (topo I) and II (topo II) relax the positive (+) and negative (-) DNA torsional stress (TS) generated ahead and behind the transcription machinery. It is unknown how this DNA relaxation activity is regulated and whether (+) and (-)TS are reduced at similar rates. Here, we used yeast circular minichromosomes to conduct the first comparative analysis of topo I and topo II activities in relaxing chromatin under (+) and (-)TS. We observed that, while topo I relaxed (+) and (-)TS with similar efficiency, topo II was more proficient and relaxed (+)TS more quickly than (-)TS. Accordingly, we found that the relaxation rate of (+)TS by endogenous topoisomerases largely surpassed that of (-)TS. We propose a model of how distinct conformations of chromatin under (+) and (-)TS may produce this unbalanced relaxation of DNA. We postulate that, while quick relaxation of (+)TS may facilitate the progression of RNA and DNA polymerases, slow relaxation of (-)TS may serve to favor DNA unwinding and other structural transitions at specific regions often required for genomic transactions.
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