Evidence map›Paper›PMID 24859755›Full record

ArticleeLife2014

Phosphoprotein SAK1 is a regulator of acclimation to singlet oxygen in Chlamydomonas reinhardtii.

Setsuko Wakao, Brian L Chin, Heidi K Ledford, Rachel M Dent, David Casero, Matteo Pellegrini, Sabeeha S Merchant, Krishna K Niyogi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 50 citations in OpenAlex.

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  13. Regulation of photoprotection gene expression inProceedings of the National Academy of Sciences of the United States of America · 2019
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Setsuko WakaoDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States.
Brian L ChinDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States.
Heidi K LedfordDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States.
Rachel M DentDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States.
David CaseroDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
Matteo PellegriniDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States Institute for Genomics and Proteomics, University of California, Los Angeles, Los Angeles, United States.
Sabeeha S MerchantInstitute for Genomics and Proteomics, University of California, Los Angeles, Los Angeles, United States Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, United States.
Krishna K NiyogiDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, United States niyogi@berkeley.edu.
University of California, Berkeley · USUniversity of California, Los Angeles · USHoward Hughes Medical Institute · US

Funding

Transcriptional profiling and annotation of the Chlamydomonas genomeR24GM092473 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MERCHANT, SABEEHA · 2011 to 2014
$1.2M
Singlet Oxygen SignalingR01GM071908 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI NIYOGI, KRISHNA K · 2004 to 2007
$1.0M
NIGMS NIH HHS R01 GM071908NIGMS NIH HHS R24 GM092473
6 · The paper itself

Abstract

Singlet oxygen is a highly toxic and inevitable byproduct of oxygenic photosynthesis. The unicellular green alga Chlamydomonas reinhardtii is capable of acclimating specifically to singlet oxygen stress, but the retrograde signaling pathway from the chloroplast to the nucleus mediating this response is unknown. Here we describe a mutant, singlet oxygen acclimation knocked-out 1 (sak1), that lacks the acclimation response to singlet oxygen. Analysis of genome-wide changes in RNA abundance during acclimation to singlet oxygen revealed that SAK1 is a key regulator of the gene expression response during acclimation. The SAK1 gene encodes an uncharacterized protein with a domain conserved among chlorophytes and present in some bZIP transcription factors. The SAK1 protein is located in the cytosol, and it is induced and phosphorylated upon exposure to singlet oxygen, suggesting that it is a critical intermediate component of the retrograde signal transduction pathway leading to singlet oxygen acclimation.DOI: http://dx.doi.org/10.7554/eLife.02286.001.

Indexed as

Adaptation, PhysiologicalChlamydomonas reinhardtiiGene Expression RegulationPhosphoproteinsSignal TransductionSinglet OxygenPhosphoproteinsSinglet Oxygenchlamydomonasphoto-oxidative stressphotosynthesisretrograde signalingsinglet oxygen

Identifiers

PMID24859755
PMCPMC4067076
OpenAlexW2171401443

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.