ArticleInsect biochemistry and molecular biology2014
High resolution genetic mapping uncovers chitin synthase-1 as the target-site of the structurally diverse mite growth inhibitors clofentezine, hexythiazox and etoxazole in Tetranychus urticae.
Article in Insect biochemistry and molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 101 citations in OpenAlex.
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- Pesticides impacts on human health and the environment with their mechanisms of action and possible countermeasures.Heliyon · 2024Review
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- Inhibition of hyaluronan secretion by novel coumarin compounds and chitin synthesis inhibitors.Glycobiology · 2021Article
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- Development of abamectin resistance in Tetranychus urticae in Australian cotton and the establishment of discriminating doses for T. lambi.Experimental & applied acarology · 2021Article
- Status and outlook for acaricide and insecticide discovery.Pest management science · 2021Review
- Acaricide resistance status and identification of resistance mutations in populations of the two-spotted spider mite Tetranychus urticae from Ethiopia.Experimental & applied acarology · 2020Article
- Botanicals AgainstPlants (Basel, Switzerland) · 2019Review
- Resistance incidence and presence of resistance mutations in populations of Tetranychus urticae from vegetable crops in Turkey.Experimental & applied acarology · 2019Article
- High-resolution QTL mapping in Tetranychus urticae reveals acaricide-specific responses and common target-site resistance after selection by different METI-I acaricides.Insect biochemistry and molecular biology · 2019Article
- Long-Term Population Studies Uncover the Genome Structure and Genetic Basis of Xenobiotic and Host Plant Adaptation in the HerbivoreGenetics · 2019Article
- Fitness costs of key point mutations that underlie acaricide target-site resistance in the two-spotted spider miteEvolutionary applications · 2018Article
- The relative contribution of target-site mutations in complex acaricide resistant phenotypes as assessed by marker assisted backcrossing in Tetranychus urticae.Scientific reports · 2017Article
- Benzoylurea resistance in western flower thripsJournal of pesticide science · 2017Article
- Disruption of a horizontally transferred phytoene desaturase abolishes carotenoid accumulation and diapause inProceedings of the National Academy of Sciences of the United States of America · 2017Article
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8 authors at 6 institutions in 6 countries.
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Abstract
The acaricides clofentezine, hexythiazox and etoxazole are commonly referred to as 'mite growth inhibitors', and clofentezine and hexythiazox have been used successfully for the integrated control of plant mite pests for decades. Although they are still important today, their mode of action has remained elusive. Recently, a mutation in chitin synthase 1 (CHS1) was linked to etoxazole resistance. In this study, we identified and investigated a Tetranychus urticae strain (HexR) harboring recessive, monogenic resistance to each of hexythiazox, clofentezine, and etoxazole. To elucidate if there is a common genetic basis for the observed cross-resistance, we adapted a previously developed bulk segregant analysis method to map with high resolution a single, shared resistance locus for all three compounds. This finding indicates that the underlying molecular basis for resistance to all three compounds is identical. This locus is centered on the CHS1 gene, and as supported by additional genetic and biochemical studies, a non-synonymous variant (I1017F) in CHS1 associates with resistance to each of the tested acaricides in HexR. Our findings thus demonstrate a shared molecular mode of action for the chemically diverse mite growth inhibitors clofentezine, hexythiazox and etoxazole as inhibitors of an essential, non-catalytic activity of CHS1. Given the previously documented cross-resistance between clofentezine, hexythiazox and the benzyolphenylurea (BPU) compounds flufenoxuron and cycloxuron, CHS1 should be also considered as a potential target-site of insecticidal BPUs.
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