ArticleThe Plant cell2014
Carotene Hydroxylase Activity Determines the Levels of Both α-Carotene and Total Carotenoids in Orange Carrots.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed, 153 citations in OpenAlex.
- Genome-Wide Characterization and Expression Analysis ofBiology · 2025Article
- A manipulation of carotenoids metabolism impacts fruit quality and drought tolerance in tomato.Plant cell reports · 2025Article
- Carotene hydroxylase DcCYP97A3 affects carotenoid metabolic flow and taproot color by influencing the conversion of α-carotene to lutein in carrot.Horticulture research · 2025Article
- Gene mapping and identification of candidate genes controlling carotenoid accumulation of yellow kernels in foxtail millet.BMC plant biology · 2025Article
- Exploring functional InDels and genetic diversity: agro-morphometric and molecular insights into the Western and Eastern gene pools of carrot (Frontiers in plant science · 2025Article
- Integrative Targeted Metabolomics and Transcriptomics Reveal the Mechanism of Leaf Coloration inInternational journal of molecular sciences · 2024Article
- Deciphering Carotenoid and Flowering Pathway Gene Variations in Eastern and Western Carrots (Genes · 2024Article
- Overexpressing CsPSY1 Gene of Tea Plant, Encoding a Phytoene Synthase, Improves α-Carotene and β-Carotene Contents in Carrot.Molecular biotechnology · 2024Article
- Chlorophyll and Carotenoid Metabolism Varies with Growth Temperatures among Tea Genotypes with Different Leaf Colors inInternational journal of molecular sciences · 2024Article
- The underlying molecular mechanisms of hormonal regulation of fruit color in fruit-bearing plants.Plant molecular biology · 2024Review
- The Y locus encodes a REPRESSOR OF PHOTOSYNTHETIC GENES protein that represses carotenoid biosynthesis via interaction with APRR2 in carrot.The Plant cell · 2024Article
- Comparative analysis of miRNA expression profiles in flowering and non-flowering tissue of Crocus sativus L.Protoplasma · 2024Article
- Article
- 15-Foods (Basel, Switzerland) · 2024Article
- The biochemical effects of carotenoids in orange carrots on the colonic proteome in a mouse model of diet-induced obesity.Frontiers in nutrition · 2024Article
- The modulation of light quality on carotenoid and tocochromanol biosynthesis in mung bean (Food chemistry. Molecular sciences · 2023Article
- Telomere-to-telomere carrot (Horticulture research · 2023Article
- Generating colorful carrot germplasm through metabolic engineering of betalains pigments.Horticulture research · 2023Article
- Transcriptome analysis reveals association of carotenoid metabolism pathway with fruit color in melon.Scientific reports · 2023Article
- Cytochrome P450 Surface Domains Prevent the β-Carotene Monohydroxylase CYP97H1 ofBiomolecules · 2023Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The typically intense carotenoid accumulation in cultivated orange-rooted carrots (Daucus carota) is determined by a high protein abundance of the rate-limiting enzyme for carotenoid biosynthesis, phytoene synthase (PSY), as compared with white-rooted cultivars. However, in contrast to other carotenoid accumulating systems, orange carrots are characterized by unusually high levels of α-carotene in addition to β-carotene. We found similarly increased α-carotene levels in leaves of orange carrots compared with white-rooted cultivars. This has also been observed in the Arabidopsis thaliana lut5 mutant carrying a defective carotene hydroxylase CYP97A3 gene. In fact, overexpression of CYP97A3 in orange carrots restored leaf carotenoid patterns almost to those found in white-rooted cultivars and strongly reduced α-carotene levels in the roots. Unexpectedly, this was accompanied by a 30 to 50% reduction in total root carotenoids and correlated with reduced PSY protein levels while PSY expression was unchanged. This suggests a negative feedback emerging from carotenoid metabolites determining PSY protein levels and, thus, total carotenoid flux. Furthermore, we identified a deficient CYP97A3 allele containing a frame-shift insertion in orange carrots. Association mapping analysis using a large carrot population revealed a significant association of this polymorphism with both α-carotene content and the α-/β-carotene ratio and explained a large proportion of the observed variation in carrots.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.