ArticleInflammation2014
Glyceraldehyde-3-phosphate dehydrogenase is an inappropriate housekeeping gene for normalising gene expression in sepsis.
Article in Inflammation, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- The molecular landscape of sepsis severity in infants: enhanced coagulation, innate immunity, and T cell repression.Frontiers in immunology · 2024Article
- Identification of autophagy-related genes and immune cell infiltration characteristics in sepsis via bioinformatic analysis.Journal of thoracic disease · 2023Article
- Systematic Identification of the Optimal Housekeeping Genes for Accurate Transcriptomic and Proteomic Profiling of Tissues following Complex Traumatic Injury.Methods and protocols · 2023Article
- Evidence-based selection of reference genes for RT-qPCR assays in periodontal research.Clinical and experimental dental research · 2022Article
- Relative versus absolute RNA quantification: a comparative analysis based on the example of endothelial expression of vasoactive receptors.Biological procedures online · 2021Article
- Chaiqin Qingning Capsule Inhibits Influenza Virus Infection and InflammationEvidence-based complementary and alternative medicine : eCAM · 2021Article
- Molecular Characterization of a Fungal Ketide Synthase Gene Among Swainsonine-Producing Alternaria Species in the USA.Current microbiology · 2020Article
- A Weak Response to Endoplasmic Reticulum Stress Is Associated With Postoperative Organ Failure in Patients Undergoing Cardiac Surgery With Cardiopulmonary Bypass.Frontiers in medicine · 2020Article
- Caspase-1 inflammasome activity in patients with Staphylococcus aureus bacteremia.Microbiology and immunology · 2019Article
- Article
- Peripheral Blood Mononuclear Cells Demonstrate Mitochondrial Damage Clearance During Sepsis.Critical care medicine · 2019Article
- Gene Expression of Protein Tyrosine Phosphatase 1B and Endoplasmic Reticulum Stress During Septic Shock.Frontiers in medicine · 2019Article
- Serum availability affects expression of common house-keeping genes in colon adenocarcinoma cell lines: implications for quantitative real-time PCR studies.Cytotechnology · 2016Article
- Distinct Profiles for Mitochondrial t-RNAs and Small Nucleolar RNAs in Locally Invasive and Metastatic Colorectal Cancer.Clinical cancer research : an official journal of the American Association for Cancer Research · 2016Article
- Gentamicin arrests cancer cell growth: the intriguing involvement of nuclear sphingomyelin metabolism.International journal of molecular sciences · 2015Article
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- Nuclear lipid microdomain as resting place of dexamethasone to impair cell proliferation.International journal of molecular sciences · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has long been used as a default reference gene in quantitative mRNA profiling experiments. However, its expression reportedly varies in response to a range of pathophysiological variables (inflammation, oxidative stress, hyperinsulinaemia, hypoxia) which feature prominently in sepsis. We therefore assessed the applicability of using GAPDH as a reference gene for expression studies in sepsis compared to other housekeeping genes (succinate dehydrogenase complex subunit A (SDHA), hypoxanthine phosphoribosyltransferase (HPRT)-1). Severe sepsis resulted in a 42.4-fold increase in median GAPDH expression (P<0.001), whereas median HPRT expression was raised more modestly (2.9-fold; P<0.001), and there was no significant difference in SDHA expression between sepsis and control patients. HPRT was identified by NormFinder to be the most stably expressed single gene. In order to assess the impact of this variability on data interpretation, interleukin (IL)-10 expression was normalised separately to GAPDH and to the geometric mean of HPRT and SDHA. In the former case, there was no significant difference in IL-10 expression between controls and septic patients, whilst in the latter, a significant 8.5-fold increase in median IL-10 expression was noted (P<0.001). GAPDH is thus an unreliable housekeeping gene for normalising gene expression in sepsis which should be replaced by alternative, validated reference genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.