ArticleDevelopmental biology2014
Integrin-α5β1 is not required for mural cell functions during development of blood vessels but is required for lymphatic-blood vessel separation and lymphovenous valve formation.
Article in Developmental biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 41 citations in OpenAlex.
- Integrins in Cardiovascular Health and Disease: Molecular Mechanisms and Therapeutic Opportunities.Biomolecules · 2025Review
- Interaction of SARS-CoV-2 with cardiomyocytes: Insight into the underlying molecular mechanisms of cardiac injury and pharmacotherapy.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2022Review
- Gene expression in meningeal lymphatic endothelial cells following traumatic brain injury in mice.PloS one · 2022Article
- The evolving cardiac lymphatic vasculature in development, repair and regeneration.Nature reviews. Cardiology · 2021Review
- Mechanisms of endothelial cell coverage by pericytes: computational modelling of cell wrapping andJournal of the Royal Society, Interface · 2020Article
- 1,25(OH)FASEB bioAdvances · 2019Article
- Establishment and maintenance of blood-lymph separation.Cellular and molecular life sciences : CMLS · 2019Review
- Lymphatic Vessel Network Structure and Physiology.Comprehensive Physiology · 2018Review
- Complementary Wnt Sources Regulate Lymphatic Vascular Development via PROX1-Dependent Wnt/β-Catenin Signaling.Cell reports · 2018Article
- Endothelium-derived fibronectin regulates neonatal vascular morphogenesis in an autocrine fashion.Angiogenesis · 2017Article
- Intraluminal valves: development, function and disease.Disease models & mechanisms · 2017Review
- Dual role of pericyte α6β1-integrin in tumour blood vessels.Journal of cell science · 2017Article
- Genetic tools for identifying and manipulating fibroblasts in the mouse.Differentiation; research in biological diversity · 2016Review
- EPHB4 kinase-inactivating mutations cause autosomal dominant lymphatic-related hydrops fetalis.The Journal of clinical investigation · 2016Article
- Mechanotransduction activates canonical Wnt/β-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves.Genes & development · 2016Article
- CHD4-regulated plasmin activation impacts lymphovenous hemostasis and hepatic vascular integrity.The Journal of clinical investigation · 2016Article
- Pdgfrb-Cre targets lymphatic endothelial cells of both venous and non-venous origins.Genesis (New York, N.Y. : 2000) · 2016Article
- Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development.Developmental biology · 2016Article
- Neural crest cell-autonomous roles of fibronectin in cardiovascular development.Development (Cambridge, England) · 2016Article
- Fibronectin signals through integrin α5β1 to regulate cardiovascular development in a cell type-specific manner.Developmental biology · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Integrin α5β1 is essential for vascular development but it remains unclear precisely where and how it functions. Here, we report that deletion of the gene encoding the integrin-α5 subunit (Itga5) using the Pdgfrb-Cre transgenic mouse line, leads to oedema, haemorrhage and increased levels of embryonic lethality. Unexpectedly, these defects were not caused by loss of α5 from Pdgfrb-Cre expressing mural cells (pericytes and vascular smooth muscle cells), which wrap around the endothelium and stabilise blood vessels, nor by defects in the heart or great vessels, but were due to abnormal development of the lymphatic vasculature. Reminiscent of the pathologies seen in the human lymphatic malformation, fetal cystic hygroma, α5 mutants display defects both in the separation of their blood and lymphatic vasculature and in the formation of the lymphovenous valves. As a consequence, α5-deficient mice develop dilated, blood-filled lymphatic vessels and lymphatic capillaries that are ectopically covered with smooth muscle cells. Analysis of the expression of Pdgfrb during lymphatic development suggests that these defects probably arise from loss of α5β1 integrin in subsets of specialised Prox1(+)Pdgfrb(+) venous endothelial cells that are essential for the separation of the jugular lymph sac from the cardinal vein and formation of the lymphovenous valve leaflets.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.