ReviewAnalytical and bioanalytical chemistry2014
Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples.
Review in Analytical and bioanalytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
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- Modern RNA Quantification Methods: From RT-qPCR to Advanced Microscopy.The journal of physical chemistry. B · 2026Review
- Overcoming Target Drift: Development and Validation of a One-Step TaqMan qPCR Assay for Epidemiological Surveillance ofMicroorganisms · 2026Article
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- When Two-Fold Is Not Enough: Quantifying Uncertainty in Low-Copy qPCR.International journal of molecular sciences · 2025Article
- MIQE 2.0 and the Urgent Need to Rethink qPCR Standards.International journal of molecular sciences · 2025Article
- Advances in nucleic acid aptamer-based detection of respiratory virus and bacteria: a mini review.Virology journal · 2024Review
- Transcriptome-guided selection of stable reference genes for expression analysis in spinach.Scientific reports · 2024Article
- Screening of reliable reference genes for the normalization of RT-qPCR in chicken liver tissues and LMH cells.Scientific reports · 2024Article
- Comparison of Cefotaxime-ResistantAntibiotics (Basel, Switzerland) · 2023Article
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- High resolution estimates of relative gene abundance with quantitative ratiometric regression PCR (qRR-PCR).The Analyst · 2021Article
- Relation between levels of toll-like receptors 3 and 7 and clinical profile of Child-Pugh B cirrhotic patients.Clinical and experimental hepatology · 2021Article
- Optimizations for identifying reference genes in bone and cartilage bioengineering.BMC biotechnology · 2021Article
- Selection of reference genes for quantitative real-time PCR normalization in European quail tissues.Molecular biology reports · 2021Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reverse transcription quantitative PCR is an established, simple and effective method for RNA measurement. However, technical standardisation challenges combined with frequent insufficient experimental detail render replication of many published findings challenging. Consequently, without adequate consideration of experimental standardisation, such findings may be sufficient for a given publication but cannot be translated to wider clinical application. This article builds on earlier standardisation work and the MIQE guidelines, discussing processes that need consideration for accurate, reproducible analysis when dealing with patient samples. By applying considerations common to the science of measurement (metrology), one can maximise the impact of gene expression studies, increasing the likelihood of their translation to clinical tools.
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