ArticleStem cell reviews and reports2014
Morphological and molecular changes of human granulosa cells exposed to 5-azacytidine and addressed toward muscular differentiation.
Article in Stem cell reviews and reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
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- Generation of Trophoblast-Like Cells From Hypomethylated Porcine Adult Dermal Fibroblasts.Frontiers in veterinary science · 2021Article
- Muscle Cell Morphogenesis, Structure, Development and Differentiation Processes Are Significantly Regulated during Human Ovarian Granulosa Cells In Vitro Cultivation.Journal of clinical medicine · 2020Article
- Adding a dimension to cell fate.Animal reproduction · 2020Article
- All roads lead to Rome: the many ways to pluripotency.Journal of assisted reproduction and genetics · 2020Review
- Expression of genes involved in neurogenesis, and neuronal precursor cell proliferation and development: Novel pathways of human ovarian granulosa cell differentiation and transdifferentiation capability in vitro.Molecular medicine reports · 2020Article
- Novel markers of human ovarian granulosa cell differentiation toward osteoblast lineage: A microarray approach.Molecular medicine reports · 2019Article
- New tools for cell reprogramming and conversion: Possible applications to livestock.Animal reproduction · 2019Article
- 'Heart development and morphogenesis' is a novel pathway for human ovarian granulosa cell differentiation during long‑term in vitro cultivation‑a microarray approach.Molecular medicine reports · 2019Article
- Genes responsible for proliferation, differentiation, and junction adhesion are significantly up-regulated in human ovarian granulosa cells during a long-term primary in vitro culture.Histochemistry and cell biology · 2019Article
- Use of a PTFE Micro-Bioreactor to Promote 3D Cell Rearrangement and Maintain High Plasticity in Epigenetically Erased Fibroblasts.Stem cell reviews and reports · 2019Article
- Epigenetic Erasing and Pancreatic Differentiation of Dermal Fibroblasts into Insulin-Producing Cells are Boosted by the Use of Low-Stiffness Substrate.Stem cell reviews and reports · 2018Article
- 5-azacytidine affects TET2 and histone transcription and reshapes morphology of human skin fibroblasts.Scientific reports · 2016Article
- Chemical transdifferentiation: closer to regenerative medicine.Frontiers of medicine · 2016Review
- Erase and Rewind: Epigenetic Conversion of Cell Fate.Stem cell reviews and reports · 2016Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Converting adult cells from one cell type to another is a particularly interesting idea for regenerative medicine. Terminally differentiated cells can be induced to de-differentiate in vitro to become multipotent progenitors. In mammals these changes do not occur naturally, however exposing differentiated adult cells to synthetic molecules capable of selectively reverting cells from their lineage commitment to a more plastic state makes it possible to re-address their fate. Only scattered information are available on the morphological changes and ultrastructural remodeling taking place when cells convert into a different and specific type. To better clarify these aspects, we derived human granulosa cell (GC) primary cultures and analyzed the morphological changes taking place in response to the exposure to the epigenetic modifier 5-azacytidine (5-aza-CR) and to the treatment with VEGF, as a stimulus for inducing differentiation into muscle cells. Ultrastructural modifications and molecular marker expression were analyzed at different intervals during the treatments. Our results indicate that the temporary up regulation of pluripotency markers is accompanied by the loss of GC-specific ultrastructural features, mainly through autophagocitosis, and is associated with a temporary chromatin decondensation. After exposure to VEGF the induction of muscle specific genes was combined with the appearance of multinucleated cells with a considerable quantity of non-spatially organized filaments. The detailed analysis of the morphological changes occurring in cells undergoing lineage re-addressing allows a better understanding of these process and may prove useful for refining the use of somatic cells in regenerative medicine and tissue replacement therapies.
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