ArticleApplied and environmental microbiology2014
The temperature gradient-forming device, an accessory unit for normal light microscopes to study the biology of hyperthermophilic microorganisms.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Article
- The use of thermostable fluorescent proteins for live imaging inFrontiers in microbiology · 2024Article
- A novel interdomain consortium from a Costa Rican oil well composed of Methanobacterium cahuitense sp. nov. and Desulfomicrobium aggregans sp. nov.Archives of microbiology · 2023Article
- The transcriptional regulator EarA and intergenic terminator sequences modulate archaellation inFrontiers in microbiology · 2023Article
- "Freezing" Thermophiles: From One Temperature Extreme to Another.Microorganisms · 2022Review
- The cell biology of archaea.Nature microbiology · 2022Review
- Life at high temperature observed in vitro upon laser heating of gold nanoparticles.Nature communications · 2022Article
- Methanofollis propanolicus sp. nov., a novel archaeal isolate from a Costa Rican oil well that uses propanol for methane production.Archives of microbiology · 2022Article
- Progress and Challenges in Archaeal Cell Biology.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Microorganisms from deep-sea hydrothermal vents.Marine life science & technology · 2021Review
- A comprehensive history of motility and Archaellation in Archaea.FEMS microbes · 2021Article
- Editorial: Astrobiology at the Interface: Interactions Between Biospheres, Geospheres, Hydrospheres and Atmospheres Under Planetary Conditions.Frontiers in microbiology · 2021Article
- The microbiomes of deep-sea hydrothermal vents: distributed globally, shaped locally.Nature reviews. Microbiology · 2019Review
- Validation of a Hypothesis: Colonization of Black Smokers by Hyperthermophilic Microorganisms.Frontiers in microbiology · 2018Article
- Motility and Ultrastructure ofFrontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To date, the behavior of hyperthermophilic microorganisms in their biotope has been studied only to a limited degree; this is especially true for motility. One reason for this lack of knowledge is the requirement for high-temperature microscopy-combined, in most cases, with the need for observations under strictly anaerobic conditions-for such studies. We have developed a custom-made, low-budget device that, for the first time, allows analyses in temperature gradients up to 40°C over a distance of just 2 cm (a biotope-relevant distance) with heating rates up to ∼5°C/s. Our temperature gradient-forming device can convert any upright light microscope into one that works at temperatures as high as 110°C. Data obtained by use of this apparatus show how very well hyperthermophiles are adapted to their biotope: they can react within seconds to elevated temperatures by starting motility-even after 9 months of storage in the cold. Using the temperature gradient-forming device, we determined the temperature ranges for swimming, and the swimming speeds, of 15 selected species of the genus Thermococcus within a few months, related these findings to the presence of cell surface appendages, and obtained the first evidence for thermotaxis in Archaea.
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